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Extracellular vesicles are pivotal in intercellular communication and hold significant promise for medical applications. However, limitations in their mass production and challenges in replicating their complex functions with artificial liposomes necessitate innovative solutions. We functionalize liposomes by combining the scalable production advantages of artificial liposomes with the vesicle fusion and formation mechanisms of bacteria. By incubating the gram-negative Shewanella oneidensis MR-1, known for its electrochemically active outer membrane cytochromes (OMCs), with liposomes containing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine for 24 hours, we achieved a substantial yield of membrane-integrated liposomes (MILs) incorporating OMCs. Circular dichroism spectroscopy confirmed the preservation of redox activity and strong inter-heme exciton coupling in the OMCs. These components were successfully delivered to Escherichia coli K-12 by incubation with MILs, retaining their functionality. Furthermore, the slow membrane exchange process did not result in cellular viability loss or lysis, allowing for the recycling of microbial cells and minimizing contaminants from lysed cells, which is advantageous for scaling up. Building on our previous work where MIL-coated titanium dioxide nanoparticles significantly enhanced radical production and effectively treated orthotopic liver tumors in vivo, our methodology to generate the MIL has promising potential to spearhead novel integrations of synthetic and biological systems for medical technologies.

権利情報:

• Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International
  

キーワード: liposome, outer-membrane vesicles, extracellular electron transport, cytochrome

刊行年月日: 2025-01-10

出版者: Elsevier BV

掲載誌:

• Nano Today (ISSN: 17480132) vol. 61 102630

研究助成金:

• Japan Society for the Promotion of Science 17H04969
• Precursory Research for Embryonic Science and Technology JPMJPR19H1
• Precursory Research for Embryonic Science and Technology P20105
• Ministry of Education MOE-109-YSFMS-1019-001-P1
• Japan Agency for Medical Research and Development 19gm6010002h0004
• Natural Science Foundation of Hunan Province 2024JJ4035
• National Science and Technology Council
• National Cheng Kung University
• Japan Science and Technology Agency
• National Science and Technology Council 112-2113-M-037-014-MY2
• National Science and Technology Council 113-2320-B-037-007-

原稿種別: 著者最終稿 (Accepted manuscript)

MDR DOI: https://doi.org/10.48505/nims.6141

公開URL: https://doi.org/10.1016/j.nantod.2025.102630

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更新時刻: 2026-01-19 09:54:02 +0900

MDRでの公開時刻: 2026-01-19 12:21:42 +0900