Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers

Gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT HAp/Col) was fabricated for anti-infection bone void fillers and was evaluated its absorption desorption properties, antibacterial efficacy and cytotoxicity. Ten milligrams of the hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder in 1 ml of the gentamicin sulfate (GNT) solution diluted with phosphate buffered saline (PBS) at 50 250 μg/ml were mixed at room temperature. After 6 h mixing, GNT adsorption in all conditions reached plateau by Langmuir’s isotherm and maximum GNT adsorption amount was 34.1±7.2 μg in 250 μg/ml GNT solution. One-hundred milligrams of the fully GNT-loaded HAp/Col powder, soaked in 10 ml of PBS at 37 °C, released all of GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to Escherichia coli at least for 1 to 2 days. Further, no cytotoxicities were observed on MG 63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresobable anti-infection bone void fillers by prevention initial infections which is primary cause of implant associate infection even for rapid bioresorbable materials.