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Description:

Here, we employ few-layer hexagonal boron nitride (hBN) as a precisely tailorable fluorescence spacer between labelled lipid membranes and graphene substrates. By stacking high-quality hBN crystals in the 10–20 nm thickness range on monolayer graphene, we observe distance-dependent fluorescence intensity variations. Remarkably, with hBN spacers as thin as 20nm, the fluorescence intensity is comparable to bare SiO2/Si substrates, while the intensity was reduced to 60 % and 80 % with ~10 nm and ~16 nm hBN thicknesses respectively. We confirm that pre- determined hBN thicknesses can be employed to control the non-radiative energy transfer properties of graphene, with fluorescence quenching following a d^-4 distance-dependent behaviour. This seamless integration of electronically active and dielectric van der Waals materials into vertical heterostructures enables multifunctional platforms addressing the manipulation, localization, and visualization of biomolecules for fundamental biophysics and biosensing applications.

Rights:

• Creative Commons BY Attribution 4.0 International
  

Keyword: Fluorescence imaging, biomolecular dynamics, hBN

Date published: 2024-03-08

Publisher: Wiley

Journal:

• ChemNanoMat (ISSN: 2199692X) vol. 10 issue. 5 e202300592

Funding:

• Nederlandse Organisatie voor Wetenschappelijk Onderzoek MechanoPore

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1002/cnma.202300592

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Updated at: 2025-02-14 12:32:02 +0900

Published on MDR: 2025-02-14 12:32:02 +0900