Article Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe

Linawati Sutrisno SAMURAI ORCID ; Gary J. Richards ORCID ; Jack D. Evans ORCID ; Michio Matsumoto SAMURAI ORCID ; Xianglan Li SAMURAI ORCID ; Koichiro Uto SAMURAI ORCID ; Jonathan P. Hill SAMURAI ORCID ; Masayasu Taki ORCID ; Shigehiro Yamaguchi ORCID ; Katsuhiko Ariga SAMURAI ORCID

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Linawati Sutrisno, Gary J. Richards, Jack D. Evans, Michio Matsumoto, Xianglan Li, Koichiro Uto, Jonathan P. Hill, Masayasu Taki, Shigehiro Yamaguchi, Katsuhiko Ariga. Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe. Science Advances. 2025, 11 (43), eadz6633. https://doi.org/10.1126/sciadv.adz6633

Description:

(abstract)

Early detection and late-stage cell fate assessment are key factors to develop therapeutic
strategies, although current methods cannot capture early responses or distinguish multiple injury
states, especially in UV-vis-sensitive cells. Here, we introduce a method to simultaneously detect
variations in RNA and DNA under near-infrared photoexcitation. Utilizing a pyrazinacene-based
probe (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including
apoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes. Specifically, TEG8-
N14 selectively stains necrotic cells in live samples, while after fixation, it allows detection of ultra-
early senescence in UV-vis-sensitive cells, providing approximately two-fold greater informational
content than existing RNA or DNA fluorophores. These findings break current imaging barriers by
enabling comprehensive visualization of single-cell fate histories without being affected by UV-vis
or genetic manipulation.

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Keyword: RNA/DNA, N-heteroacene, senescence, near-infrared, UV-vis sensitive, Mutiplex labeling, Bioimaging

Date published: 2025-10-24

Publisher: American Association for the Advancement of Science (AAAS)

Journal:

  • Science Advances (ISSN: 23752548) vol. 11 issue. 43 eadz6633

Funding:

  • JSPS JP24KF0089
  • JSPS JP25K23571
  • JSPS JP21K05044
  • Australian Research Council Discovery Early Career Award DE220100163

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1126/sciadv.adz6633

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Updated at: 2025-10-28 12:30:18 +0900

Published on MDR: 2025-10-28 12:16:23 +0900

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