Download file (3.87MB)
Download as zip (3.17MB)

コレクション

引用

説明:

Early detection and late-stage cell fate assessment are key factors to develop therapeutic
strategies, although current methods cannot capture early responses or distinguish multiple injury
states, especially in UV-vis-sensitive cells. Here, we introduce a method to simultaneously detect
variations in RNA and DNA under near-infrared photoexcitation. Utilizing a pyrazinacene-based
probe (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including
apoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes. Specifically, TEG8-
N14 selectively stains necrotic cells in live samples, while after fixation, it allows detection of ultra-
early senescence in UV-vis-sensitive cells, providing approximately two-fold greater informational
content than existing RNA or DNA fluorophores. These findings break current imaging barriers by
enabling comprehensive visualization of single-cell fate histories without being affected by UV-vis
or genetic manipulation.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: RNA/DNA, N-heteroacene, senescence, near-infrared, UV-vis sensitive, Mutiplex labeling, Bioimaging

刊行年月日: 2025-10-24

出版者: American Association for the Advancement of Science (AAAS)

掲載誌:

• Science Advances (ISSN: 23752548) vol. 11 issue. 43 eadz6633

研究助成金:

• JSPS JP24KF0089
• JSPS JP25K23571
• JSPS JP21K05044
• Australian Research Council Discovery Early Career Award DE220100163

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1126/sciadv.adz6633

関連資料:

その他の識別子:

連絡先:

更新時刻: 2025-10-28 12:30:18 +0900

MDRでの公開時刻: 2025-10-28 12:16:23 +0900