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説明:

Methods to encode digital data items as strands of synthetic DNA followed by selective data retrieval have been demonstrated. However, these initially bio-oriented processes remain slow and not optimized. DNA field-effect transistor (DNA-FET) is studied here as a possible random-access memory (RAM) device for simple, selective and rapid ssDNA fragment retrieval used as data pool identifier. The DNA-FET is based on a co-planar Au-gated fully organic transistor appended with short single-stranded DNA (ssDNA) probes bearing a blocking molecule to prevent partial hybridization and achieve near perfect selectivity for short length ssDNA (up to 45 nt). Examination of transconductance of the novel active layer incorporating a DNA nanopore architecture reveals enhanced binding site accessibility. This, in turn, facilitates discriminatory hybridization, particularly in the physical retrieval of short-length ssDNA from a competitive, concentrated ssDNA background pool consisting of nine different sequences, with at least one nucleotide difference. The DNA-FET exhibits rapid operation (9 min) in the millivolt range, low detection limit (sub-femtomolar), high selectivity and reusability. Considering the straightforward concept, near error-free identification
capacity and hypothetically outstanding scalability, the DNA-FET described here has potential as a foundation for further exploration of advanced RAM technology in the DNA data storage process.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: DNA, Field effect transistor, Random access memory

刊行年月日: 2024-02-02

出版者: Wiley

掲載誌:

• Advanced Sensor Research (ISSN: 27511219) vol. 3 issue. 5 p. 1-14 2300176

研究助成金:

• Japan Society for the Promotion of Science
• Ministry of Education, Culture, Sports, Science and Technology

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/adsr.202300176

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更新時刻: 2024-08-19 16:30:24 +0900

MDRでの公開時刻: 2024-08-19 16:30:24 +0900