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Description:

A fluid-based scaffold with tunable viscoelasticity has been developed to investigate its impact on the cell adhesion process. The platform is based on the water–perfluorocarbon interface decorated with diacetylene-based phospholipid membranes (IPLMs), whose viscoelasticity can be systematically manipulated by photocrosslinking. Further introduction of a cell-adhesive peptide and fluorescent tag allows cell adhesion at the highly deformable fluid interface and confocal observation of dynamic cell–model ECM interactions. Cells seeded at the IPLM exhibit so-called adaptive wetting, where the interface first deforms toward the out-of-plane direction before cellular dimensional changes, followed by cellular flattening and interfacial restoration. Furthermore, the quantification of these parameters reveals a biphasic response against the crosslinking levels, which indicates that the cell-ECM viscosity balance determines adaptive wetting phenotypes.

Rights:

• Creative Commons BY Attribution 4.0 International
  

Keyword: Mechanobiology, Cell Adhesion, Fluid, Cell Scaffold, Viscoelasticity

Date published: 2025-01-16

Publisher: Wiley

Journal:

• Advanced Functional Materials (ISSN: 1616301X) vol. 35 issue. 7

Funding:

• Japan Society for the Promotion of Science 22H00596
• Japan Society for the Promotion of Science 23K17481
• Japan Society for the Promotion of Science 23KJ2167

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1002/adfm.202414534

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Updated at: 2025-06-10 12:30:22 +0900

Published on MDR: 2025-06-10 12:22:44 +0900