Download file (4.29MB)
Download as zip (3.84MB)

コレクション

引用

説明:

A fluid-based scaffold with tunable viscoelasticity has been developed to investigate its impact on the cell adhesion process. The platform is based on the water–perfluorocarbon interface decorated with diacetylene-based phospholipid membranes (IPLMs), whose viscoelasticity can be systematically manipulated by photocrosslinking. Further introduction of a cell-adhesive peptide and fluorescent tag allows cell adhesion at the highly deformable fluid interface and confocal observation of dynamic cell–model ECM interactions. Cells seeded at the IPLM exhibit so-called adaptive wetting, where the interface first deforms toward the out-of-plane direction before cellular dimensional changes, followed by cellular flattening and interfacial restoration. Furthermore, the quantification of these parameters reveals a biphasic response against the crosslinking levels, which indicates that the cell-ECM viscosity balance determines adaptive wetting phenotypes.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Mechanobiology, Cell Adhesion, Fluid, Cell Scaffold, Viscoelasticity

刊行年月日: 2025-01-16

出版者: Wiley

掲載誌:

• Advanced Functional Materials (ISSN: 1616301X) vol. 35 issue. 7

研究助成金:

• Japan Society for the Promotion of Science 22H00596
• Japan Society for the Promotion of Science 23K17481
• Japan Society for the Promotion of Science 23KJ2167

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/adfm.202414534

関連資料:

その他の識別子:

連絡先:

更新時刻: 2025-06-10 12:30:22 +0900

MDRでの公開時刻: 2025-06-10 12:22:44 +0900