Article CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance Separation of Human Mesenchymal Stromal Cells

Moe Kato ; Tadashi Nakaji ; Kazuaki Matsumura ; Chiaki Yoshikawa SAMURAI ORCID (National Institute for Materials Science) ; Yuki Usui ; Takahiro Kishioka ; Taito Nishino

ChemBioChem - 2026 - Kato - CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance (1).pdf
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Moe Kato, Tadashi Nakaji, Kazuaki Matsumura, Chiaki Yoshikawa, Yuki Usui, Takahiro Kishioka, Taito Nishino. CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance Separation of Human Mesenchymal Stromal Cells. CHEMBIOCHEM. 2026, 27 (3), . https://doi.org/10.1002/cbic.202500822

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(abstract)

Cell transplantation therapy represents a promising strategy in next-generation regenerative medicine; however, its clinical application remains limited by the absence of safe and label-free methods for obtaining pure populations of functional cells. This study reports a peptide-functionalized zwitterionic polymer system designed for the selective and non-invasive isolation of human mesenchymal stem cells (hMSCs) from heterogeneous cell populations. The substrate was modified with poly(CMBMAm-co-PGMAn-co-MPTMS1) (PCmPnM1), synthesized via free radical polymerization and subsequently conjugated with a CD44-binding peptide (CD44BP). Among the compositions examined, the PC7P2M1–CD44BP surface exhibited superior antifouling properties, effectively suppressing nonspecific protein adsorption and adhesion of non-target cells while selectively capturing hMSCs. A column packed with PC7P2M1–CD44BP–modified silica microparticles successfully isolated high-purity hMSCs from mixed cell suspensions within 35 minutes. Flow cytometry confirmed that the later-eluted cells exhibited higher CD44 and CD105 expression, indicating separation based on antigen expression. The isolated hMSCs maintained proliferation and differentiation capacities equivalent to those of pre-separated cells, demonstrating that this device preserves cell functionality. This peptide–polymer hybrid column provides a simple and clinically adaptable platform for safe, label-free purification of hMSCs intended for cell transplantation therapy.

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Keyword: antifouling, cell separation, hMSC, label-free purification

Date published: 2026-02-12

Publisher: Wiley

Journal:

  • CHEMBIOCHEM (ISSN: 14394227) vol. 27 issue. 3

Funding:

  • Japan Society for the Promotion of Science

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1002/cbic.202500822

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Updated at: 2026-02-27 08:30:32 +0900

Published on MDR: 2026-02-26 17:51:09 +0900

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