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The advent of single-molecule atomic-resolution time-resolved electron microscopy (SMART-EM) has created a new field of “cinematic chemistry,” allowing for the cinematographic recording of dynamic behaviors of organic and inorganic molecules and their assembly. However, the limited electron dose per frame of video images presents a major challenge in SMART-EM. Recent advances in direct electron counting cameras and techniques to enhance image quality through the implementation of a denoising algorithm have enabled the tracking of stochastic molecular motions and chemical reactions with sub-millisecond temporal resolution and sub-angstrom localization precision. This review showcases the development of dynamic molecular imaging using the SMART-EM technique, highlighting insights into nanomechanical behavior during molecular shuttle motion, pathways of multistep chemical reactions, and elucidation of crystallization processes at the atomic level.

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  This is a pre-copyedited, author-produced version of an article accepted for publication in Microscopy following peer review. The version of record Koji Harano, Takayuki Nakamuro, Eiichi Nakamura, Cinematographic study of stochastic chemical events at atomic resolution, Microscopy, Volume 73, Issue 2, April 2024, Pages 101–116, is available online at: https://doi.org/10.1093/jmicro/dfad052

Keyword: SMART-EM, in-situ imaging, transmission electron microscopy, time-resolved imaging

Date published: 2024-04-08

Publisher: Oxford University Press (OUP)

Journal:

• Microscopy (ISSN: 00220744) vol. 73 issue. 2 p. 101-116

Funding:

• Japan Science and Technology Agency JPMJSN16B1
• Japan Society for the Promotion of Science JP19H05459 JP21H01758 JP22K14704 JP23H04874
• Core Research for Evolutional Science and Technology JPMJCR20B2
• Japan Science and Technology Agency JPMJSN16B1
• Japan Society for the Promotion of Science JP19H05459 JP21H01758 JP22K14704 JP23H04874
• Core Research for Evolutional Science and Technology JPMJCR20B2

Manuscript type: Author's version (Accepted manuscript)

MDR DOI: https://doi.org/10.48505/nims.4659

First published URL: https://doi.org/10.1093/jmicro/dfad052

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Updated at: 2024-10-21 12:30:22 +0900

Published on MDR: 2024-10-21 12:30:24 +0900