Article Ionic Liquid Interface as a Cell Scaffold

Takeshi Ueki SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS)) ; Koichiro Uto SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS)) ; Shota Yamamoto SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS)) ; Ryota Tamate SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS)) ; Yuji Kamiyama ORCID ; Xiaofang Jia ORCID ; Hidenori Noguchi SAMURAI ORCID (Research Center for Energy and Environmental Materials (GREEN), National Institute for Materials Science (NIMS)) ; Kosuke Minami SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS)) ; Katsuhiko Ariga SAMURAI ORCID (Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS)) ; Hongxin Wang SAMURAI ORCID ; Jun Nakanishi SAMURAI ORCID (Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS))

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Citation
Takeshi Ueki, Koichiro Uto, Shota Yamamoto, Ryota Tamate, Yuji Kamiyama, Xiaofang Jia, Hidenori Noguchi, Kosuke Minami, Katsuhiko Ariga, Hongxin Wang, Jun Nakanishi. Ionic Liquid Interface as a Cell Scaffold. Advanced Materials. 2024, (), 2310105. https://doi.org/10.1002/adma.202310105
SAMURAI

Description:

(abstract)

In sharp contrast to conventional solid/hydrogel platforms, water-immiscible liquids, such as perfluorocarbons and silicones, allow the adhesion of mammalian cells via protein nanolayers (PNLs) formed at the interface. However, fluorocarbons and silicones, which are typically used for liquid cell culture, possess only narrow ranges of physicochemical parameters and have not allowed for a wide variety of cell culturing environments. In this paper, it is proposed that water-immiscible ionic liquids (ILs) are a new family of liquid substrates with tunable physicochemical properties and high solvation capabilities. Tetraalkylphosphonium-based ILs are identified as non-cytotoxic ILs, whereon human mesenchymal stem cells are successfully cultured. By reducing the cation charge distribution, or ionicity, via alkyl chain elongation, the interface allows cell spreading with matured focal contacts. High-speed atomic force microscopy observations of the PNL formation process suggest that the cation charge distribution significantly altered the protein adsorption dynamics, which are associated with the degree of protein denaturation and the PNL mechanics. Moreover, by exploiting dissolution capability of ILs, anion-gel cell scaffold is fabricated. This enables to further identify the significant contribution of bulk subphase mechanics to cellular mechanosensing in liquid-based culture scaffolds.

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Keyword: ionic liquid, cell culture, mechanobiology, gels, liquid interface, stem cell, phosphonium

Date published: 2024-02-26

Publisher: Wiley

Journal:

  • Advanced Materials (ISSN: 09359648) 2310105

Funding:

  • JSPS 20H02804 (to T.U.)
  • JSPS 20K21229 (to T.U.)
  • JSPS 23H02030 (to T.U.)
  • JSPS 22K14705 (to S.Y.)
  • JSPS 22H00596 (to J.N.)
  • JSPS 23K17418 (to J.N.)

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1002/adma.202310105

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Updated at: 2024-03-05 08:30:08 +0900

Published on MDR: 2024-03-05 08:30:08 +0900

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