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In sharp contrast to conventional solid/hydrogel platforms, water-immiscible liquids, such as perfluorocarbons and silicones, allow the adhesion of mammalian cells via protein nanolayers (PNLs) formed at the interface. However, fluorocarbons and silicones, which are typically used for liquid cell culture, possess only narrow ranges of physicochemical parameters and have not allowed for a wide variety of cell culturing environments. In this paper, it is proposed that water-immiscible ionic liquids (ILs) are a new family of liquid substrates with tunable physicochemical properties and high solvation capabilities. Tetraalkylphosphonium-based ILs are identified as non-cytotoxic ILs, whereon human mesenchymal stem cells are successfully cultured. By reducing the cation charge distribution, or ionicity, via alkyl chain elongation, the interface allows cell spreading with matured focal contacts. High-speed atomic force microscopy observations of the PNL formation process suggest that the cation charge distribution significantly altered the protein adsorption dynamics, which are associated with the degree of protein denaturation and the PNL mechanics. Moreover, by exploiting dissolution capability of ILs, anion-gel cell scaffold is fabricated. This enables to further identify the significant contribution of bulk subphase mechanics to cellular mechanosensing in liquid-based culture scaffolds.

権利情報:

• In Copyright
  

キーワード: ionic liquid, cell culture, mechanobiology, gels, liquid interface, stem cell, phosphonium

刊行年月日: 2024-02-26

出版者: Wiley

掲載誌:

• Advanced Materials (ISSN: 09359648) 2310105

研究助成金:

• JSPS 20H02804 (to T.U.)
• JSPS 20K21229 (to T.U.)
• JSPS 23H02030 (to T.U.)
• JSPS 22K14705 (to S.Y.)
• JSPS 22H00596 (to J.N.)
• JSPS 23K17418 (to J.N.)

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/adma.202310105

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更新時刻: 2024-03-05 08:30:08 +0900

MDRでの公開時刻: 2024-03-05 08:30:08 +0900