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This article outlines the preparation of three distinct surface-charged SiQDs-F127 (+0.03, + 9.13, -7.05 mV), varying from negative to positive charges. Depending on the surface charges, the amount of CpG ODNs also varies; positively charged materials take up more ODNs (-44.31 mV) than neutral (-37.10 mV) and negatively charged (-30.00 mV) materials due to electrostatic attraction. We assessed the viability of the cells, as well as their uptake and localization in endosomes. PSiQDs with a positive charge are more localized and induce higher levels of cytokines, including IL-6 and IFN-α, due to increased endosomal localization compared to other surface-charged PSiQDs. The results indicate that the surface charges of the nanoparticle play a significant role in delivering CpG ODN2006x3-PD and inducing cytokines.

Rights:

• Creative Commons BY Attribution 4.0 International
  

Keyword: Colloidal Quantum dots, Bioimaging, Silicon nanocrystals, CpG oligodeoxynucleotides, Immune-stimulation, Pluronic F127

Date published: 2024-04-26

Publisher: Springer Science and Business Media LLC

Journal:

• Scientific Reports (ISSN: 20452322) vol. 14 9618

Funding:

• MEXT | Japan Society for the Promotion of Science 22K19291
• MEXT | Japan Society for the Promotion of Science 23H02607

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1038/s41598-024-60536-2

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Updated at: 2024-05-01 08:30:14 +0900

Published on MDR: 2024-05-01 08:30:14 +0900