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Description:

Pancreatic islet transplantation is one of the clinical options for certain types of diabetes. However, difficulty in maintaining islets prior to transplantation limits the clinical expansion of islet transplantations. Our study introduces a dynamic culture platform developed specifically for primary human islets by mimicking the physiological microenvironment, including tissue fluidics and extracellular matrix support. We engineered the dynamic culture system by incorporating our distinctive microwell-patterned porous collagen scaffolds for loading isolated human islets, enabling vertical medium flow through the scaffolds. The physiological microenvironment-mimetic culture platform supported the viability and quality of isolated human islets at high-seeding density.

Rights:

• Creative Commons BY-NC Attribution-NonCommercial 4.0 International
  

Keyword: pancreatic islets, collagen scaffold, dynamic culture, hypoxia, physiomimetic culture

Date published: 2024-05-14

Publisher: SAGE Publications

Journal:

• Cell Transplantation (ISSN: 09636897) vol. 33 p. 49556-49556 49556

Funding:

• National Institutes of Health P30CA033572
• National Institutes of Health R03DK129958-01
• Japan Society for the Promotion of Science 22K19926
• Nora Eccles Treadwell Foundation
• JDRF 3-SRA-2021-1073-S-B
• National Cancer Institute of the National Institutes of Health P30CA033572

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1177/09636897241249556

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Updated at: 2024-08-05 16:30:28 +0900

Published on MDR: 2024-08-05 16:30:28 +0900