Visualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual RNA/DNA Targeting Probe

SUTRISNO Linawati

Poster Visualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual RNA/DNA Targeting Probe

SUTRISNO Linawati (International Center for Young Scientists, National Institute for Materials Science)

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SUTRISNO Linawati. Visualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual RNA/DNA Targeting Probe. https://doi.org/10.48505/nims.5850

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Early detection and late-stage cell fate assessment are key factors to develop therapeutic strategies, although current methods cannot capture early responses or distinguish multiple injury states, especially in UV-vis-sensitive cells. Here, we introduce a method to simultaneously detect variations in RNA and DNA under near-infrared photoexcitation. Utilizing a pyrazinacene-based probe (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including apoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes. Specifically, TEG8-N14 selectively stains necrotic cells in live samples, while after fixation, it allows detection of ultra-early senescence in UV-vis-sensitive cells, providing approximately two-fold greater informational content than existing RNA or DNA fluorophores. These findings break current imaging barriers by enabling comprehensive visualization of single-cell fate histories without being affected by UV-vis or genetic manipulation.

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