Significance of homogeneous operation in light-assisted fixed-bed bioprocess under ammonia stress: Optimization, long-term operation and metagenomic analysis

Yunxin Zhu; YuJia Chen; Guangqi An; Cheng Zhang; Jingwei Yang; Rongyong Yang; Guoping Chen; Yingnan Yang

doi:10.1016/j.biortech.2024.130568

論文 Significance of homogeneous operation in light-assisted fixed-bed bioprocess under ammonia stress: Optimization, long-term operation and metagenomic analysis

Yunxin Zhu (School of Life and Environmental Sciences Degree Programs in Life and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba) ; YuJia Chen (School of Life and Environmental Sciences Degree Programs in Life and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba) ; Guangqi An (Research Center for Macromolecules and Biomaterials/Biomaterials Field/Tissue Regeneration Materials Group, National Institute for Materials Science) ; Cheng Zhang (School of Life and Environmental Sciences Degree Programs in Life and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba) ; Jingwei Yang (School of Life and Environmental Sciences Degree Programs in Life and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba) ; Rongyong Yang (Shanghai High Victory Science and Technology Co., Ltd.) ; Guoping Chen (Research Center for Macromolecules and Biomaterials/Biomaterials Field/Tissue Regeneration Materials Group, National Institute for Materials Science) ; Yingnan Yang (School of Life and Environmental Sciences Degree Programs in Life and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba)

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Yunxin Zhu, YuJia Chen, Guangqi An, Cheng Zhang, Jingwei Yang, Rongyong Yang, Guoping Chen, Yingnan Yang. Significance of homogeneous operation in light-assisted fixed-bed bioprocess under ammonia stress: Optimization, long-term operation and metagenomic analysis. BIORESOURCE TECHNOLOGY. 2024, 399 (), 130568. https://doi.org/10.1016/j.biortech.2024.130568

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(abstract)

Activating microbes with light is a promising strategy for addressing ammonia-stressed anaerobic digestion (AD). However, as a critical in-process parameter, homogenous operation, in light-assisted AD amended by bio-fixed bed has received limited attention. This research endeavors to establish a uniform-illuminated biosystem and assess its practical feasibility through a 90-day semi-continuous operation at pilot scale under solar light illumination. With optimal stirring mode (intermittent stirring for 3 min every 15 min), robust methane yields were achieved across various organic loads, reaching 88.7–94.3% of theoretical yield under high ammonium stress (3500 mg/L). The metagenomic analysis unveiled that uniform illumination triggered synergistic effects in AD, fostering a diversified microbial consortium, enhancing carbohydrate and methane metabolism, and facilitating the formation of an electroactive bio-cluster. This study underscores the significance of homogenous illumination in AD systems for efficient waste-to-energy conversion, highlighting the implementation of solar light as a greener approach for scale-up application.

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