Article A Novel Gene Synthesis Platform for Designing Functional Protein Polymers

Toshimasa Homma ; Rie Yamamoto ; Lily Zuin Ping Ang ; Alaa Fehaid SAMURAI ORCID (National Institute for Materials Science) ; Mitsuhiro Ebara SAMURAI ORCID (National Institute for Materials Science)

25_AdvSci.pdf
Download file (2.12 MB)
Download as zip (1.81 MB)
Collection

Citation
Toshimasa Homma, Rie Yamamoto, Lily Zuin Ping Ang, Alaa Fehaid, Mitsuhiro Ebara. A Novel Gene Synthesis Platform for Designing Functional Protein Polymers. Advanced Science. 2025, 12 (15), 2410903. https://doi.org/10.1002/advs.202410903

Description:

(abstract)

In this study, a novel method is proposed herein: seamless cloning of rolling-circle amplicons (SCRCA). This method involves one-pot preparation of repetitive-sequence genes with overlapping ends for cloning, facilitating the easy construction of the desired recombinants. SCRCA is used to synthesize 10 genes encoding hydrophilic resilin-like and hydrophobic elastin-like repeat units that induce liquid-liquid phase separation.

Rights:

Keyword: Gene synthesis, Protein Polymers

Date published: 2025-02-23

Publisher: Wiley

Journal:

  • Advanced Science (ISSN: 21983844) vol. 12 issue. 15 2410903

Funding:

  • New Energy and Industrial Technology Development Organization JPNP20004
  • Ministry of Agriculture, Forestry and Fisheries JP22680575
  • National Institute for Materials Science AM2100
  • Japan Society for the Promotion of Science 22K14549
  • Japan Society for the Promotion of Science 24H01378

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1002/advs.202410903

Related item:

Other identifier(s):

Contact agent:

Updated at: 2026-01-06 15:59:19 +0900

Published on MDR: 2026-01-07 08:24:29 +0900

Filename Size
Filename 25_AdvSci.pdf (Thumbnail)
application/pdf 		Size 2.12 MB Detail