Download file (2.12MB)
Download as zip (1.81MB)

コレクション

引用

説明:

In this study, a novel method is proposed herein: seamless cloning of rolling-circle amplicons (SCRCA). This method involves one-pot preparation of repetitive-sequence genes with overlapping ends for cloning, facilitating the easy construction of the desired recombinants. SCRCA is used to synthesize 10 genes encoding hydrophilic resilin-like and hydrophobic elastin-like repeat units that induce liquid-liquid phase separation.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Gene synthesis, Protein Polymers

刊行年月日: 2025-02-23

出版者: Wiley

掲載誌:

• Advanced Science (ISSN: 21983844) vol. 12 issue. 15 2410903

研究助成金:

• New Energy and Industrial Technology Development Organization JPNP20004
• Ministry of Agriculture, Forestry and Fisheries JP22680575
• National Institute for Materials Science AM2100
• Japan Society for the Promotion of Science 22K14549
• Japan Society for the Promotion of Science 24H01378

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/advs.202410903

関連資料:

その他の識別子:

連絡先:

更新時刻: 2026-01-06 15:59:19 +0900

MDRでの公開時刻: 2026-01-07 08:24:29 +0900