Article Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells

Mazaya Najmina (National Institute for Materials Science) ; Nicholaus Kevin Tanjaya SAMURAI ORCID (National Institute for Materials Science) ; Satoshi Ishii SAMURAI ORCID (National Institute for Materials Science) ; Koichiro Uto SAMURAI ORCID (National Institute for Materials Science)

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Mazaya Najmina, Nicholaus Kevin Tanjaya, Satoshi Ishii, Koichiro Uto. Label-free classification of growth-arrested state of cells by quantitative phase microscopy in fixative-treated cells. Science and Technology of Advanced Materials: Methods. 2025, 5 (1), 2525061. https://doi.org/10.1080/27660400.2025.2525061

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(abstract)

In the current work, we take advantage of protein accumulation to distinguish senescence cells from other cells. The protein concentration is visualized by recording the phase difference using the quantitative phase microscopy (QPM). Our live QPM observations on breast cancer cells confirms that senescent cells exhibit a higher refractive index compared to cells in normal growth and quiescent states, attributed to the accumulation of undigested lysosomal protein cargo.

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Keyword: Label-free classification, Growth-arrested state, Quantitative phase microscopy

Date published: 2025-12-31

Publisher: Informa UK Limited

Journal:

  • Science and Technology of Advanced Materials: Methods (ISSN: 27660400) vol. 5 issue. 1 2525061

Funding:

  • JST FOREST Program JPMJFR2327
  • Grant-in-Aid for Transformative Research Areas JP20H05877
  • Japan Society for the Promotion of Science

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1080/27660400.2025.2525061

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Updated at: 2025-08-04 12:30:27 +0900

Published on MDR: 2025-08-04 12:17:08 +0900

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