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Description:

As a stimulus-responsive drug release system, we developed Diels–Alder (DA) induction-activated magnetic nanoparticles (DiMaN). Functionalization of polymer-coated magnetic nanoparticles (mNPs) with drugs via thermoreversible DA coupling enabled release through retro-Diels–Alder (rDA) cleavage upon heating. The (pDMAm-co-pFMA)-b-pAAc polymer supported mNP formation and drug binding. DA coupling with maleimide-functionalized drugs was verified by 1H NMR, showing distinct exo (3.23 ppm) and endo (3.48 ppm) signals after 72 h at 37 °C. Approximately 70% release was observed within 15 min at 80 °C, while the complex maintained stability at 40 °C. The superparamagnetic mNPs generated localized heating under an alternating current magnetic field (192 kHz, 480 A), raising the solution temperature by 6 °C within 5 min. The biotin-maleimide complex demonstrated higher release by rDA from furan-containing mNPs (approximately 150 μM) compared to the control group (approximately 103 μM). These results highlight DiMaN as a promising platform for magnetic-controlled, on-demand drug release.

Rights:

• Creative Commons BY Attribution 4.0 International
  

Keyword: Diels-Alder reaction, magnetic hyperthermia, on-demand

Date published: 2025-11-10

Publisher: American Chemical Society (ACS)

Journal:

• Biomacromolecules (ISSN: 15257797) vol. 26 issue. 11 p. 7265-7274

Funding:

• Ministry of Education, Culture, Sports, Science and Technology JPMXP1224NM5127
• National Institute of Allergy and Infectious Diseases AI163282
• Japan Society for the Promotion of Science 20H05877
• Japan Society for the Promotion of Science 22KJ0434

Manuscript type: Publisher's version (Version of record)

MDR DOI:

First published URL: https://doi.org/10.1021/acs.biomac.5c00321

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Updated at: 2025-12-19 08:30:20 +0900

Published on MDR: 2025-12-18 19:08:21 +0900