# Fileset

[MaterTrans67(2026)83-91_suppl.pdf](https://mdr.nims.go.jp/filesets/f91c3219-f9a9-4ba3-986a-983cb22ef3f9/download)

## Creator

[Akiko Yamamoto](https://orcid.org/0000-0002-9182-4886), Yuki Ito

## Rights

[In Copyright](http://rightsstatements.org/vocab/InC/1.0/)

## Other metadata

[Effect of Humidity on Antibacterial Activity of Copper and Its Alloy Surfaces](https://mdr.nims.go.jp/datasets/67a1bc3e-028f-424d-a5f7-8437fa51e591)

## Fulltext

PowerPoint プレゼンテーションSupplementary materials for the manuscript entitled“Effect of humidity on antibacterial activity of copper and its alloy surfaces”which is authored by Akiko Yamamoto and Yuki Ito.Eight pages with following figures and tables:Figure S1; Schematic explanation of the antibacterial test procedure using WST-1 including data analysis for the estimation of initial number of survived bacterial cells.Figure S2; Additional antibacterial test results of C7060, C7150 and MONEL 400 by the film method.Figure S3; Additional antibacterial test results of MONEL 400 and antibacterial stainless steel under controlled humidity (90% RH).Table S1; The results of the statistical analysis of the antibacterial assay under controlled humidity for C1020 with Escherichia coli by parallelism test of 2 regression lines (data in Fig. 2).Table S2; The results of the statistical analysis of the antibacterial assay under controlled humidity of 60%RH with Escherichia coli by parallelism test of 2 regression lines (data in Fig. 3a).Table S3; The results of the statistical analysis of the antibacterial assay under controlled humidity of 35%RH with Escherichia coli by parallelism test of 2 regression lines (data in Fig. 3b).Table S4; The results of the statistical analysis of the antibacterial assay under controlled humidity of 60%RH with Staphylococcus aureus by parallelism test of 2 regression lines (data in Fig. 4a).Table S5. The results of the statistical analysis of the antibacterial assay under controlled humidity of 35%RH with Staphylococcus aureus by parallelism test of 2 regression lines (data in Fig. 4b).Fig.S1 Schematic explanation of (a) procedure of antibacterial activity evaluation using WST-1, (b) absorbance change obtained by periodical measurement, and (c) calibrationcurve plotting the time to reach the absorbance 0.5 and the inoculated number ofbacteria.ca.1x105 CFU/1µLin phosphate buffer with 0.5% Tween 80Swab 20 times each100 times in totalAdd 480μL of NB, 30 sec of vortex mixingpolyester bud with 20 μL of Nutrient Broth(NB)Collect 180 μLAdd 20 μL of the mixture of 5mM WST-1 and 0.2mM 1-methoxy-PMS soln.Incubate @ 35˚C up to 16~24hAbsorbance @450nmIncubate @25ºC, 35-90%RH, 5min~24h(a)0.11100 4 8 12 16Asample-AblankIncubation period(h)4200000420000420004200420424.2(b)4.2106CFU/mL4.21054.21044.21034.21024.21014.2100(c)y = -0.6ln(x) + 12.439R² = 0.99790510151.E+0 1.E+1 1.E+2 1.E+3 1.E+4 1.E+5 1.E+6 1.E+7T Abs0.5(h)Inoculated no. of bacteria (CFU/mL)100   101   102   103   104   105   106   1070.0010.010.111 10 100 1000Bacterial viabilityContact time (min)E.coliC7060C7150MONEL(a)0.0010.010.111 10 100 1000Bacterial viabilityContact time (min)S.aureusC7060C7150MONELFig.S2 The results of antibacterial assay by the film method for C7060, C7150, andMONEL against Escherichia coli (a) and Staphylococcus aureus (b), mean±sd.(b)S10.00010.0010.010.11101 10 100 1000Bacterial viabiiltyContact time (min)MONEL_90%RHMONEL_60%RHMONEL_35%RHE. coli(a)(b)0.000010.00010.0010.010.11101 10 100 1000 10000Bacterial viabilityContact time (min)ABSS_90%RHABSS_60%RHABSS_35%RHE. coliFig.S3 The results of the antibacterial assay under controlled humidity for MONEL(a) and antibacterial stainless steel (b) against Escherichia coli. N=2, mean±sd.10110010-110-210-310-410110010-110-210-310-410-5S275%RH 60%RH 50%RH 35%RH90%RH Not Sig. p<0.01, slope p<0.001, slope p<0.001, slope75%RH — p<0.001, slope p<0.001, slope p<0.001, slope60%RH — — p<0.001, slope p<0.001, slope50%RH — — — p<0.001, interceptTable S1. The results of the statistical analysis of the antibacterial assay undercontrolled humidity for C1020 with Escherichia coli by parallelism test of 2regression lines (data in Fig. 2).“Slope” indicates the null hypothesis of the equality in the slopes of two regression lines.“Intercept” indicates the null hypothesis of the equality in the intercepts of two regression lines, which have the equal slope.C7060 C7150 MONEL ABSSC1020 p<0.01, intercept p<0.05, slope p<0.001, slope p<0.001, slopeC7060 — p<0.001, intercept p<0.001, slope p<0.001, slopeC7150 — — p<0.001, slope p<0.01, slopeMONEL — — — p<0.01, slopeTable S2. The results of the statistical analysis of the antibacterial assay undercontrolled humidity of 60%RH with Escherichia coli by parallelism test of 2regression lines (data in Fig. 3a).“Slope” indicates the null hypothesis of the equality in the slopes of two regression lines.“Intercept” indicates the null hypothesis of the equality in the intercepts of two regression lines, which have the equal slope.C7060 C7150 MONEL ABSSC1020 p<0.001, intercept p<0.05, intercept p<0.001, intercept p<0.01, slopeC7060 — p<0.001, intercept p<0.001, intercept p<0.05, slopeC7150 — — p<0.05, slope p<0.001, slopeMONEL — — — p<0.05, slopeTable S3. The results of the statistical analysis of the antibacterial assay undercontrolled humidity of 35%RH with Escherichia coli by parallelism test of 2regression lines (data in Fig. 3b).“Slope” indicates the null hypothesis of the equality in the slopes of two regression lines.“Intercept” indicates the null hypothesis of the equality in the intercepts of two regression lines, which have the equal slope.C7060 C7150 MONEL ABSSC1020 p<0.01, slope p<0.001, slope p<0.001, slope p<0.01, interceptC7060 — p<0.001, slope p<0.001, slope p<0.001, slopeC7150 — — p<0.001, slope p<0.001, slopeMONEL — — — p<0.001, slopeTable S4. The results of the statistical analysis of the antibacterial assay undercontrolled humidity of 60%RH with Staphylococcus aureus by parallelism test of 2regression lines (data in Fig. 4a).“Slope” indicates the null hypothesis of the equality in the slopes of two regression lines.“Intercept” indicates the null hypothesis of the equality in the intercepts of two regression lines, which have the equal slope.C7060 C7150 MONEL ABSSC1020 p<0.001, intercept p<0.001, intercept p<0.001, intercept p<0.01, interceptC7060 — Not Sig. p<0.001, intercept p<0.001, interceptC7150 — — p<0.05, slope p<0.01, slopeMONEL — — — p<0.05, interceptTable S5. The results of the statistical analysis of the antibacterial assay undercontrolled humidity of 35%RH with Staphylococcus aureus by parallelism test of 2regression lines (data in Fig. 4b).“Slope” indicates the null hypothesis of the equality in the slopes of two regression lines.“Intercept” indicates the null hypothesis of the equality in the intercepts of two regression lines, which have the equal slope. スライド 1 スライド 2 スライド 3 スライド 4 スライド 5 スライド 6 スライド 7 スライド 8 スライド 9