# Fileset

[Poster.pdf](https://mdr.nims.go.jp/filesets/c423f3e5-9e72-4c02-8b38-3e8e5cae4be1/download)

## Creator

[SUTRISNO Linawati](https://orcid.org/0000-0003-3085-9660)

## Rights

[Creative Commons BY Attribution 4.0 International](https://creativecommons.org/licenses/by/4.0/)

## Other metadata

[Visualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual RNA/DNA Targeting Probe](https://mdr.nims.go.jp/datasets/367e06c6-a2c0-4271-88d5-9104cc715c15)

## Fulltext

Presentation TitleVisualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual-RNA/DNA-Targeting Probe1International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS); 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanLinawati Sutrisno1Science Advances, Accepted (2025)Research backgroundPrevious work on the development of pyrazinacene as a chromophoreE-mail: SUTRISNO.Linawati@nims.go.jpResearch purposeKey questions about DNA and RNA in aging cells are still underexplored → long-term imaging is crucial for spatiotemporal mapping of cell fate transitionsacenePoor stabilityEnhance stabilityN6 N8 N10 N12 N14Red-shift absorption wavelength• Hill, et al. Acc. Chem. Res. 2021, 54, 16, 3228-3240. • Hill, et al. J. Am. Chem. Soc. 2019, 141, 19570−19574.Acid/base responsiveness in organic solvents850800750700650600550H+H-H+H-Fluorescence emissionThe longest known pyrazinacene (N14)'s protonation influences its near-infrared (NIR) fluorescence, offering potential for long-term imaging+Inaccurate results → has been ignored over decadesReveal uncovered biological  phenomenon in photoaged cells using modified  pyrazinacene (N14) for bioimaging UVA UVBProblems in visualizing DNA-RNA interactionImpaired extracellular matrices → aging MMP-1Tavernarakis et al. Nat. Aging 2023, 3, 34-46. -Nucleotide excision repairDetection of cell state based on DNA-RNACheckpoint signalingDNA mutation ?STOPS G2MG1ATTAA A CT T GCGCancerDNA damage responseLimited channel for DNA-RNA interactionHigh autofluorescenceLow photostability or toxicFixation → RNA degradationLive FixExcitation <532 nm↓ RNA selectivityDevelopment of pyrazinacenesN14Provide crucial biological insights, as well as new drug developmentDNA damageUnique: dual labeling of RNA and DNANucleusNucleoliDNA-RNA interaction?Ex<650 nmEm<720 nmEx>690 nmEm>720 nmRNA (nucleolus)RNA (cytoplasm)DNA (nucleus)RNA (nucleolus)RNA (cytoplasm) DNA (nucleus)RNA (nucleolus)RNA (cytoplasm)DNA (nucleus)Magenta ch.Cyan ch.MergeN14 N14Merge10 µmHigh RNA-DNA specificityCorrelation plotMagenta ch.N14Blue ch.Hoechst 33342Correlation plotR: 0.88Green ch.Nucleolus Bright GreenCyan ch.N14R: 0.96Senescence (aging) detectionProliferativeFully senescenceSPIDER β-Gal(β-galactosidase)This work: OKConventional method: inaccurate (exc.< 640 nm)AutofluorescenceN14 (RNA/DNA)Autofluorescence + SPIDER β-gal SummaryConventional approach to stain RNA/DNA UV-Vis sensitive cells Cannot distinguish states     or long-term imaging  Single organelle stain   Cannot detect multiple      cell statesThis work: First exploration of N14 for bioimaging✓ Quantify multiple cell stage✓ UV-Vis sensitive cells✓ Multiplex and long-term imaging  Acridine Orange (AO)PC5 trans-1-Halo10 µm20 µm10 µmMulticolor imagingFunding: Japan Society for the Promotion of Science (JSPS) KAKENHI grant numbers JP24KF0089 and JP25K23571                 Australian Research Council Discovery Early Career Award (project number: DE220100163)  Slide 1: Visualizing the Chronicle of Multiple Cell Fates using New Class of Near-IR Dual-RNA/DNA-Targeting Probe