# Fileset

[Ag-HAp_Col 41598_2026_54878_MOESM1_ESM.pdf](https://mdr.nims.go.jp/filesets/b740786c-5104-42ab-a3da-65fd85d2903d/download)

## Creator

[Masanori Kikuchi](https://orcid.org/0000-0002-9451-8147), Taku Yoshida, [Yasushi Suetsugu](https://orcid.org/0000-0002-8161-1908), [Akiko Yamamoto](https://orcid.org/0000-0002-9182-4886)

## Rights

[Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International](https://creativecommons.org/licenses/by-nc-nd/4.0/)

## Other metadata

[Preparation and antibacterial activities of silver nanoparticle-loaded hydroxyapatite/collagen bone-like nanocomposite](https://mdr.nims.go.jp/datasets/42cfe2e9-d4d0-4249-a4c1-7bd8d7bf4711)

## Fulltext

41598_2026_54878_MOESM3_ESMSupporting Information for the manuscripts entitled  “Preparation and Antibacterial Activities of Silver Nanoparticle-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite” authored by Masanori Kikuchi et al.  1. Details of the pseudo-first order model (PFO model) and the pseudo-second order model (PSO model) for adsorption equilibrium analysis.  2. Four major, two-parameter adsorption isotherm models  3. Supplementary Figure S1: Schematic drawing of donut-shaped HCM for antibacterial and cytotoxicity tests.  4. Supplementary Figure S2: Schematic explanation of (a) absorbance change obtained by periodical measurement, and (b) calibration curve plotting the time to reach the absorbance 1.0 and the inoculated number of bacteria.  5. Supplementary Figure S3: Powder X-ray diffraction (XRD) pattern of HAp/Col. Annotations are Miller indices for HAp. Collagen cannot be detected on XRD.  6. Supplementary Figure S4: Fourier-transformed infrared spectrum of HAp/Col. Small amount of carbonate group was substituted in PO43– sites of HAp.  Five pages (including this cover page) with 4 figures.   1. Details of the pseudo-first order model and pseudo-second order model for absorption equilibrium time analysis  The pseudo-first order model (PFO model) [18] and the pseudo-second order model (PSO model) [19] described as following equations: Kinetics curve of PFO model 𝑄! = 𝑄"{1 − exp(−𝑘#𝑡)} … (1) Kinetics curve of PSO model 𝑄! =𝑘$𝑄"$𝑡𝑘$𝑄"𝑡 + 1 … (2) where t: time elapsed from the start of adsorption,  Qt: adsorption amounts at each period (mg•g–1), Qe: equilibrium adsorption amount (mg•g–1), k1: adsorption rate constant for PFO model (min–1), k2: adsorption rate constant for PSO model (g•mg–1•min–1). Transform the above equations: ln(𝑄" − 𝑄!) = ln(𝑄") − 𝑘#𝑡 … (1)% 𝑡𝑄!=1𝑘$𝑄"$ +𝑡𝑄"…(2)%. Based on these equations, t vs ln(Qe–Qt) and t vs t/Qt were plotted to calculate linear fitting curves and parameters for the appropriate model.   2. Details of 4 major, two-parameter adsorption isotherm models  The details of 4 major, two-parameter adsorption isotherm models, Langmuir, Freundlich, Dubinin-Radushkevich (D-R), and Temkin, are described as follows [21]: Langmuir isotherm model 𝑄" =𝑄&𝐾'𝐶"1 + 𝐾'𝐶"…(3) 1𝑄"=1𝑄&+1𝑄&𝐾'𝐶"…(3)% Freundlich isotherm model 𝑄" = 𝐾(𝐶"#)  … (4) 𝑙𝑛 𝑄" = 𝑙𝑛𝐾( +1𝑛 𝑙𝑛 𝐶" …(4)% D-R isotherm model 𝑄" = 𝑄* exp (−𝐾+,𝜀$) … (5) ε = 𝑅𝑇 𝑙𝑛 @1 +1𝐶"A … (6) 𝑙𝑛 𝑄" = 𝑙𝑛𝑄* − 𝐾+,𝜀$…(5)% Temkin isotherm model 𝑄" = 𝐵 𝑙𝑛(𝐴-𝐶") … (7) 𝐵 =𝑅𝑇𝐾-…(8) 𝑄" = 𝐵 𝑙𝑛𝐴- + 𝐵 𝑙𝑛𝐶" …(7)% where Ce: equilibrium concentration (mg•L–1) Qe: equilibrium adsorption amount (mg•g–1) KL: Langmuir constant (L•mg–1) Q0: maximum adsorption amount of monolayer (mg•g–1) Kf: Freundlich constant (L•mg–1) n: heterogeneity in the adsorption process Kdr ∶ D-R constant (mol2•kJ–2) ε∶ the Polanyi potential (kJ•mol–1) Qs ∶ theoretical saturation capacity (mg•g–1) R∶ universal gas constant (8.314 J•mol–1•K‒1) T∶ Kelvin temperature (K) AT ∶ the equilibrium binding constant (L•g–1) B∶ the constant related to the heat of adsorption (J•mol–1) KT ∶ Temkin constant   Figure S1. Schematic drawing of donut-shaped HCM for antibacterial and cytotoxicity tests.     Figure S2. Schematic explanation of (a) absorbance change obtained by periodical measurement, and (b) calibration curve plotting the time to reach the absorbance 1.0 and the inoculated number of bacteria.     Figure S3. Powder X-ray diffraction (XRD) pattern of HAp/Col. Annotations are Miller indices for HAp. Collagen cannot be detected on XRD.      (a) (b)100.010.11Acontrol -Ablankt / h 10–11061051041031021011000 108642 12TAbs1.0 / h CFU / mL1061051041031021011003 10864 5 97y = 2•10   •e12 -2.911xR  = 0.99412 Figure S4: Fourier-transformed infrared spectrum of HAp/Col. Small amount of carbonate group was substituted in PO43– sites of HAp.