# Fileset

[adom202400868-sup-0001-suppmat.docx](https://mdr.nims.go.jp/filesets/7c7e3400-29f3-46b1-a076-fc666b872e2b/download)

## Creator

Qi Hu, [Masanobu Iwanaga](https://orcid.org/0000-0002-8930-6940), Youhong Tang

## Rights

[Creative Commons BY Attribution 4.0 International](https://creativecommons.org/licenses/by/4.0/)

## Other metadata

[Metasurface Platform Incorporating Aggregation Induced Emission Based Biosensor for Enhanced Human Serum Albumin Detection](https://mdr.nims.go.jp/datasets/bc1d2a52-50fe-499f-9c87-f52dfe613188)

## Fulltext

Metasurface Platform Incorporating Aggregation Induced Emission Based Biosensor for Enhanced Human Serum Albumin DetectionQi Hu 1, 2, Masanobu Iwanaga 1, *, Youhong Tang 2, *1. Research Centre for Electronic and Optical Materials, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan2. Institute for NanoScale Science and Technology, Medical Device Research Institute, College of Science and Engineering, Flinders university, South Australia 5042, Australia* Corresponding authors. iwanaga.masanobu@nims.go.jp (M Iwanaga) and youhong.tang@flinders.edu.au (Y Tang)Supporting informationFigure S1. (A) and (B) Electric and magnetic field intensities (|E|2 and |H|2), respectively, induced at 750 nm, which is indicated by an arrow in Figure 2B. This xz-section was set to cut through the centre of the Si nanocolumns. Incident field was set to be unity, that is, |Ein|2 = 1 and |Hin|2 = 1. Colour bars indicate the field intensities. White lines show the interfaces of the Si nanocolumns with air and the underlying SiO2 layer.ABFigure S2. (A) and (B) Simulated and measured reflectance spectra, respectively. Figure S3. The effect of reaction sequence between different combinations among biotin-HSA antibody (HSA Ab), HSA, and TPE-4TA (4TA); Brackets represent a well-mixed solution that have been fully reacted and incubated in advance with these components. [HSA] = 5 μg mL-1, [Biotin-HSA antibody] = 5 μg mL-1, [TPE-4TA] = 20 μg mL-1, Exposure time: 6000 ms, Gain: 10.Figure S4. Explanation for FL quenching effect.Figure S5. The change of FL efficiency with different concentrations of HL555-HSA Ab; [Biotin-HSA Ab] = 2 μg mL-1, Exposure time: 3000 ms, Gain: 10. Inset figure represents FL quenching before and after adding HSA. [Biotin-HSA Ab] = 2 μg mL-1, [HL555-HSA Ab] = 5 μg mL-1, Exposure time: 3000 ms, Gain: 10. Figure S6. The FL spectra of TPE-4TA+HSA (black), TPE-4TA (red), HSA (blue) and PBS (green). λex = 350 nm, [HSA] = 100 μg mL-1 and [TPE-4TA] = 10 μg mL-1.Figure S7. TPE-4TA concentration effect with or without HSA; [TPE-4TA] = 0-20 μg mL-1, [HSA] = 0, 100, 1000 μg mL-1, Exposure time: 3000 ms, Gain: 10.Figure S8. The standard curve of TPE-4TA for HSA detection using the microplate platform. λex = 360 nm, [HSA] = 0-200 μg mL-1 and [TPE-4TA] = 20 μg mL-1, Exposure time: 3000ms, Gain: 10.Figure S9. The standard curve of TPE-4TA for HSA detection using the microfluidic platform. λex = 360 nm, [HSA] = 0-200 μg mL-1 and [TPE-4TA] = 20 μg mL-1, Exposure time: 3000ms, Gain: 10.Figure S10. Spectrum of untreated urine. Figure S11. The effect of urine dilution where dilution ratios refer to total volume/treated urine volume with PBS buffer as diluent. λex = 360 nm, Exposure time: 3000ms, Gain: 10.  Content Commercialkits Microplate wells system Microfluidic system Metasurface platform Measured concentration 45.828 μg mL-1 40.720 μg mL-1 41.917 μg mL-1 43.832 μg mL-1 Recovery rate ─── 88.85% 91.47% 95.64%Table S1.The measurement of HSA concentrations and corresponding recovery rates in urine among microplate, microfluidic and metasurface platforms.8image3.emf1100 1000 900 800 700 600 500 400 300Wavelength (nm)100806040200Reflectance (%)s pol, θ=5 degMeasuredimage4.emf1100 1000 900 800 700 600 500 400 300Wavelength (nm)100806040200Reflectance (%)s pol, θ=5 degSimulatedimage5.emf1100 1000 900 800 700 600 500 400 300Wavelength (nm)100806040200Reflectance (%)s pol, θ=5 degMeasuredimage6.emfPBS+4TAHSA Ab+4TA HSA+4TA HSA Ab+(HSA+4TA) (HSA Ab+ HSA)+4TA (HSA Ab+HSA+4TA)0.91.01.11.2I/I0image7.pngimage8.pngimage9.emf350 400 450 500 550 600 650 7000100020003000400050006000700080009000FL intensity (a.u.)Wavelength (nm) TPE-4TA+HSA TPE-4TA HSA PBSimage10.emf0 5 10 15 2001000020000300004000050000 TPE-4TA with 1000 μg/mL HSA TPE-4TA with 100 μg/mL HSA TPE-4TA without HSAI-I0(a.u.)[TPE-4TA] (μg/mL)image11.emf0 50 100 150 200050001000015000200002500030000I-I0(a.u.)HSA Concentration (μg/mL)Equationy = a + b*xIntercept1522.53844 ± 812.2Slope138.57356 ± 9.9487Adj. R-Squa0.965image12.emf0 50 100 150 200050010001500200025003000350040004500I-I0(a.u.)HSA Concentration (μg/mL)Equationy = a + b*xIntercept397.77798 ± 132.960Slope18.90714 ± 1.44015Adj. R-Squar0.97165image13.emf300 400 500 600 700 8000200400600800100012001400FL intensity(a.u.)Wavelength (nm) Excitation Emission364 nm444 nmimage14.emfUndiluted 5x 10x 20x 40x 80x 160x PBS02000400060008000100001200014000FL intensity (a.u.)image1.pngimage2.emf1100 1000 900 800 700 600 500 400 300Wavelength (nm)100806040200Reflectance (%)s pol, θ=5 degSimulated