# Fileset

[Revised_Supp_for_STAM.pdf](https://mdr.nims.go.jp/filesets/6bb2a9f4-7792-40d1-a5d2-f049cbc9d9b2/download)

## Creator

Riki Toita, Yuki Shimizu, Jeong-Hun Kang

## Rights

[Creative Commons BY Attribution 4.0 International](https://creativecommons.org/licenses/by/4.0/)

## Other metadata

[Distinct macrophage uptake of engineered and biological particles driven by host age and sex](https://mdr.nims.go.jp/datasets/bbabbd10-f452-4980-ae0f-615e38ca6acd)

## Fulltext

S1  Supporting Information  Distinct macrophage uptake of engineered and biological particles driven by host age and sex Riki Toitaa, 1, *, Yuki Shimizub, 1, Jeong-Hun Kangc a Molecular Biosystems Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan; b Molecular Biosystems Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan; c National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan; 1 These authors contributed equally to this study.  *Corresponding author: Riki Toita, Ph.D.  Molecular Biosystems Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Osaka, Japan. E-mail: toita-r@aist.go.jp           S2    Figure S1. CD11b immunostaining of bone marrow-derived macrophages. After fixation with 4% paraformaldehyde in PBS, cells were stained with an Alexa Fluor 488–conjugated anti-mouse/human CD11b antibody (clone M1/70; 1:100; BioLegend, San Diego, CA, USA), and nuclei were counterstained with Hoechst 33342 (1:500; Dojindo, Kumamoto, Japan). Images were acquired using a ZOE Fluorescence Cell Imager (Bio-Rad Laboratories, Hercules, CA, USA). Brightness was adjusted uniformly across all images using PowerPoint. S3    Figure S2. Standard slopes of fluorophore-modified polymeric particles.            S4   Figure S3. Standard slopes of fluorescein-modified bioparticles.                            S5    Figure S4. Amount of particle uptake 4 h after exposure of macrophages to particles.          S6    Figure S5. Principal component analysis (PCA). (A) PCA of raw count data before batch correction. (B) PCA after batch correction using ComBat-seq, showing mitigation of batch effects across samples and identification of a potential outlier sample.       S7    Figure S6. Blocking the MARCO receptor with an anti-MARCO antibody reduces the uptake of L-COOH particles by bone marrow-derived macrophages. Y, young; O, old; M, male; F, female. Data are means ± SE (n = 5). Statistical significance was determined by paired t-test. *p < 0.05; ****p < 0.0001; ns, not significant.                S8  Table S1. List of common DEGs showing sex-dependent differences in macrophages.  High in female (8 genes) Low in female (83 genes)     C1qtnf6 Eif2s3y Col5a1 Cnn3 Pde1c Ddx3y Col16a1 Thbs2 Eif2s3x Kdm5d Sema7a Col8a1 Padi4 Uty Map1b Amotl2 Odf3l1 Gm29650 Phldb2 Col12a1 Cpne8 ENSMUSG00000121547 Col3a1 Fn1 Gdf3 Grem2 Cyp1b1 Timp3 Xist Cd248 Sparc Fermt2   Vgll3 Col5a2 Ccn2   Ccn5 Cxcl12 Grb10   Eml1 Acta2 Dpysl3   Loxl1 Fstl1 Lamb1   Nid1 Col6a1 Tgfb1i1   Tjp1 Col5a3 Mxra8   Itga11 Loxl2 Parva   Col1a1 Fat1 Cemip   Actg2 Fkbp10 Fscn1   Hspg2 Fmod Msrb3   Postn Mrc2 Aebp1   Ddr2 Bgn Bmp1   Col6a3 Serpinh1 Tead1   Igfbp7 Adam12 Ccdc80   Pxdn Fbn1 Enah   Crlf1 Cald1 Timp1   Col1a2 Myl9 Tnc   Podnl1 Ccn4 Ltbp1   Fbln2 Cdh11 Inhba   Cdh2 Adamts2