# Fileset

[bmmad7085supp1.docx](https://mdr.nims.go.jp/filesets/2985dcad-599f-4b50-8ef4-408ccd267766/download)

## Creator

[Yu Yusa](https://orcid.org/0009-0009-1395-8182), Yoshinaka Shimizu, Masanobu Hayashi, [Takayuki Aizawa](https://orcid.org/0000-0002-6231-0368), Takahiro Nakahara, Takahiro Ueno, Akimitsu Sato, Chieko Miura, [Akiko Yamamoto](https://orcid.org/0000-0002-9182-4886), Yoshimichi Imai

## Rights

Original content from this work may be used under the terms of the
Creative Commons Attribution 4.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal
citation and DOI.[Creative Commons BY Attribution 4.0 International](https://creativecommons.org/licenses/by/4.0/)

## Other metadata

[Effect of hematoma on early degradation behavior of magnesium after implantation](https://mdr.nims.go.jp/datasets/5f5b5bd9-776b-4bab-9176-9cd264495e81)

## Fulltext

Table S1. Weight loss of the implanted magnesium specimen after acid cleaning (mean ± SD, µg/mm2).  day 1 day 3 day 7 Non-hematoma group 4.41 ± 1.22 10.01 ± 3.38 14.77 ± 3.28 Hematoma group 6.10 ± 1.03 17.28 ± 3.21 27.31 ± 5.91 Statistical significance* p<0.05 p<0.01 p<0.01*The statistical significance between the two groups on each implantation period was decided by t-test.Table S2. Weight gain as insoluble salts after implantation (mean ± SD) and its ratio against the weight loss decided after acid cleaning.  weight (µg/mm2) ratio to weight loss (%)  day 1 day 3 day 7 day 1 day 3 day 7 Non-hematoma group 3.89 ± 1.30 8.89 ± 2.95 13.28 ± 3.15 86.8 ± 9.54 88.9 ± 5.65 89.8 ± 4.62 Hematoma group 8.15 ± 3.43 13.37 ± 2.46 18.62 ± 2.31 135.3 ± 58.9 77.4 ± 3.75 69.4 ± 7.87 Statistical significance* p<0.05 p<0.05 p<0.01 p>0.1 p<0.05 p<0.05*The statistical significance between the two groups on each implantation period was decided by t-test.Table S3. Weight of Mg in insoluble salts quantified in the collected acid mixture (mean ± SD) and its ratio to the total weight of the insoluble salts.  weight (µg/mm2) ratio to insoluble salt weight (%)  day 1 day 3 day 7 day 1 day 3 day 7 Non-hematoma group 0.710 ± 0.236 1.546 ± 0.608 2.303 ± 0.268 18.6 ± 3.19 17.2 ± 2.05 18.0 ± 3.82 Hematoma group 1.527 ± 0.619 2.233 ± 0.601 3.248 ± 0.377 19.0 ± 2.29 16.6 ± 2.50 17.5 ± 1.48 Statistical significance* p<0.05 p=0.08 p<0.01 p>0.1 p>0.1 p>0.1*The statistical significance between the two groups on each implantation period was decided by t-test.Table S4. Weight of Ca in insoluble salts quantified in the collected acid mixture (mean ± SD) and its ratio to the total weight of the insoluble salts.  weight (µg/mm2) ratio to insoluble salt weight  day 1 day 3 day 7 day 1 day 3 day 7 Non-hematoma group 0.683 ± 0.324 1.098 ± 0.764 1.419 ± 0.435 18.9 ± 9.37 12.7 ± 6.64 11.2 ± 4.82 Hematoma group 0.420 ± 0.174 0.875 ± 0.198 1.406 ± 0.289 5.97 ± 3.13 6.64 ± 0.951 7.51 ± 0.886 Statistical significance* p>0.1 p>0.1 p>0.1 p<0.05 P=0.08 p>0.1*The statistical significance between the two groups on each implantation period was decided by t-test.Table S5. Weight of P in insoluble salts quantified in the collected acid mixture (mean ± SD) and its ratio to the total weight of the insoluble salts.  weight (µg/mm2) ratio to insoluble salt weight (%)  day 1 day 3 day 7 day 1 day 3 day 7 Non-hematoma group 0.614 ± 0.084 0.950 ± 0.208 1.511 ± 0.239 17.1 ± 4.60 11.2 ± 2.24 11.8 ± 2.52 Hematoma group 0.377 ± 0.094 0.883 ± 0.217 1.505 ± 0.267 4.60 ± 2.30 6.71 ± 1.68 8.04±5.35 Statistical significance* p<0.01 p>0.1 p>0.1 p<0.01 p<0.01 p<0.05*The statistical significance between the two groups on each implantation period was decided by t-test.Table S6. Results of EDX analysis of pure Mg specimens implanted under non-hematoma and hematoma conditions (atm. %).  Non-hematoma Hematoma  p   day 1 day 3 day 7 day 1 day 3 day 7 day 1 day 3 day 7 Mg 24.2 ± 5.4 8.9 ± 0.3 7.9 ± 1. 6 42.3 ± 2.7 12.8 ± 1.3 6.2 ± 0.4 <0.05 <0.05 >0.1 O 50.2 ± 2.2 60.9 ± 0.2 55.0 ± 8.5 30.1 ± 1.8 62.0 ± 3.4 51.0 ± 8.3 <0.01 >0.1 >0.1 Ca 1.3 ± 0.4 4.2 ± 0.2 4.5 ± 0.2 0.4 ± 0.0 1.0 ± 0.1 2.2 ± 0.8 <0.05 <0.01 <0.05 P 4.6 ± 0.2 8.2 ± 0.4 7.6 ± 0.3 1.3 ± 0.0 2.6 ± 0.5 4.2 ± 1.1 <0.01 <0.01 <0.05 C 19.7 ± 2.8 17.8 ± 0.9 25.1 ± 9.9 25.9 ± 1.3 21.6 ± 2.9 36.4 ± 9.6 <0.05 >0.1 >0.1*The statistical significance between the two groups on each implantation period was decided by t-test.(a)(b)X-ray energy (kev)Counts20000040008000120001600010 µmCaOCPMgFigure S1. Typical examples of the EDX analysis (a) of pure Mg specimen surface before implantation. The EDX spectrum (b) was obtained by the point analysis of a red square indicated in the SEM image in (a).Counts  Figure S2. Typical examples of the EDX analysis (a) of pure Mg specimen surface implanted for 3 days under non-hematoma condition. The EDX spectrum (b) was obtained by the point analysis of a red square indicated in the SEM image in (a).(b)(a)X-ray energy (kev)CountsCaOCPMg05001000150020002500300010 µmCountsFigure S3. Typical examples of the EDX analysis (a) of pure Mg specimen surface implanted for 7 days under non-hematoma condition. The EDX spectrum (b) was obtained by the point analysis of a red square indicated in the SEM image in (a).(a)CaOCPMg0100020003000400050006000X-ray energy (kev)Counts10 µm(b)CountsFigure S4. Typical examples of the EDX analysis (a) of pure Mg specimen surface implanted for 3 days under hematoma condition. The EDX spectrum (b) was obtained by the point analysis of a red square indicated in the SEM image in (a).(a)X-ray energy (kev)CountsCaOCPMg50000100020003000400010 µm(b)Counts(a)CaOCPMg0250050007500100001250015000CountsX-ray energy (kev)10 µm(b) Figure S5. Typical examples of the EDX analysis (a) of pure Mg specimen surface implanted for 7 days under hematoma condition. The EDX spectrum (b) was obtained by the point analysis of a red square indicated in the SEM image in (a).Raman intensityRaman shift (cm-1)Figure S6. Raman spectra of the pure Mg specimen surface before implantation.-0.93379999999999996 1.9116 4.7560000000000002 7.5994999999999999 10.4422 13.283799999999999 16.124600000000001 18.964400000000001 21.8033 24.641200000000001 27.478200000000001 30.314299999999999 33.149500000000003 35.983800000000002 38.817100000000003 41.649500000000003 44.481000000000002 47.311500000000002 50.141100000000002 52.969799999999999 55.797600000000003 58.624400000000001 61.450400000000002 64.275400000000005 67.099500000000006 69.922600000000003 72.744900000000001 75.566199999999995 78.386600000000001 81.206100000000006 84.024699999999996 86.842299999999994 89.659000000000006 92.474800000000002 95.289699999999996 98.103700000000003 100.91679999999999 103.7289 106.5401 109.3505 112.15989999999999 114.9683 117.77589999999999 120.5826 123.3883 126.1932 128.99709999999999 131.80009999999999 134.60220000000001 137.4034 140.2037 143.00299999999999 145.8015 148.59899999999999 151.39570000000001 154.19139999999999 156.9863 159.78020000000001 162.57320000000001 165.36529999999999 168.15649999999999 170.9468 173.7362 176.5247 179.31229999999999 182.09899999999999 184.88470000000001 187.6696 190.45359999999999 193.23670000000001 196.0188 198.80009999999999 201.5805 204.35990000000001 207.13849999999999 209.9162 212.69300000000001 215.46879999999999 218.24379999999999 221.0179 223.7911 226.5634 229.3347 232.1052 234.87479999999999 237.64359999999999 240.41139999999999 243.17830000000001 245.9443 248.70939999999999 251.47370000000001 254.23699999999999 256.99950000000001 259.76100000000002 262.52170000000001 265.28149999999999 268.04039999999998 270.79840000000002 273.55549999999999 276.31169999999997 279.06700000000001 281.82150000000001 284.57499999999999 287.32769999999999 290.0795 292.8304 295.5804 298.3295 301.07780000000002 303.82510000000002 306.57159999999999 309.31720000000001 312.06189999999998 314.80579999999998 317.5487 320.29070000000002 323.03190000000001 325.7722 328.51159999999999 331.25020000000001 333.98779999999999 336.72460000000001 339.46050000000002 342.19549999999998 344.92970000000003 347.66289999999998 350.39530000000002 353.1268 355.85750000000002 358.5872 361.31610000000001 364.04410000000001 366.77120000000002 369.4975 372.22289999999998 374.94740000000002 377.67099999999999 380.3938 383.1157 385.83670000000001 388.55689999999998 391.27609999999999 393.99450000000002 396.71210000000002 399.42869999999999 402.14449999999999 404.85950000000003 407.57350000000002 410.2867 412.9991 415.71050000000002 418.42110000000002 421.13080000000002 423.83969999999999 426.54770000000002 429.25479999999999 431.96109999999999 434.66640000000001 437.37099999999998 440.07459999999998 442.7774 445.4794 448.18049999999999 450.88069999999999 453.58 456.27850000000001 458.97620000000001 461.67290000000003 464.3689 467.06389999999999 469.75810000000001 472.45139999999998 475.14400000000001 477.8356 480.52629999999999 483.21620000000001 485.90530000000001 488.59350000000001 491.2808 493.96730000000002 496.65289999999999 499.33769999999998 502.02159999999998 504.7047 507.38690000000003 510.06830000000002 512.74879999999996 515.42840000000001 518.10720000000003 520.78520000000003 523.46230000000003 526.13850000000002 528.81389999999999 531.48850000000004 534.16219999999998 536.83500000000004 539.50699999999995 542.17819999999995 544.84839999999997 547.51790000000005 550.18650000000002 552.85429999999997 555.52120000000002 558.18730000000005 560.85249999999996 563.51689999999996 566.18050000000005 568.84310000000005 571.505 574.16600000000005 576.82619999999997 579.4855 582.14400000000001 584.80160000000001 587.45839999999998 590.11440000000005 592.76949999999999 595.42380000000003 598.07719999999995 600.72979999999995 603.38149999999996 606.03250000000003 608.68259999999998 611.33180000000004 613.98019999999997 616.6277 619.27449999999999 621.9203 624.56539999999995 627.2097 629.85299999999995 632.49559999999997 635.13729999999998 637.77819999999997 640.41830000000004 643.0575 645.69590000000005 648.33339999999998 650.97019999999998 653.60599999999999 656.24109999999996 658.87530000000004 661.50869999999998 664.1413 666.77300000000002 669.40390000000002 672.03399999999999 674.66330000000005 677.29169999999999 679.91930000000002 682.54610000000002 685.17200000000003 687.7971 690.42139999999995 693.04489999999998 695.66750000000002 698.28930000000003 700.91030000000001 703.53049999999996 706.14980000000003 708.76829999999995 711.38599999999997 714.00289999999995 716.61900000000003 719.23419999999999 721.84860000000003 724.46220000000005 727.07500000000005 729.68690000000004 732.298 734.90830000000005 737.51779999999997 740.12649999999996 742.73429999999996 745.34140000000002 747.94759999999997 750.553 753.15750000000003 755.76130000000001 758.36429999999996 760.96640000000002 763.56769999999995 766.16819999999996 768.76790000000005 771.36680000000001 773.96479999999997 776.56209999999999 779.1585 781.75409999999999 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925.82339999999999 928.37329999999997 930.92240000000004 933.47080000000005 936.01829999999995 938.56510000000003 941.11099999999999 943.65620000000001 946.20060000000001 948.74419999999998 951.28700000000003 953.82899999999995 956.37019999999995 958.91070000000002 961.45039999999995 963.98929999999996 966.52729999999997 969.06460000000004 971.60109999999997 974.13689999999997 976.67179999999996 979.20600000000002 981.73940000000005 984.27200000000005 986.80380000000002 989.33479999999997 991.86509999999998 994.39449999999999 996.92319999999995 999.4511 1001.9783 1004.5046 1007.0302 1009.5549 1012.0789 1014.6022 1017.1246 1019.6463 1022.1672 1024.6873000000001 1027.2067 1029.7252000000001 1032.2429 1034.76 1037.2762 1039.7916 1042.3063 1044.8202000000001 1047.3334 1049.8457000000001 1052.3572999999999 1054.8679999999999 1057.3782000000001 1059.8875 1062.3959 1064.9036000000001 1067.4105 1069.9167 1072.4221 1074.9268 1077.4306999999999 1079.9337 1082.4359999999999 1084.9376 1087.4384 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1591.4103 1593.7577000000001 1596.1045999999999 1598.4507000000001 1600.7961 1603.1409000000001 1605.4848999999999 1607.8280999999999 1610.1708000000001 1612.5127 1614.854 1617.1945000000001 1619.5343 1621.8733999999999 1624.2119 1626.5497 1628.8867 1631.2230999999999 1633.5587 1635.8938000000001 1638.2280000000001 1640.5616 1642.8945000000001 1645.2266999999999 1647.5581999999999 1649.8889999999999 1652.2191 1654.5486000000001 1656.8773000000001 1659.2053000000001 1661.5327 1663.8594000000001 1666.1854000000001 1668.5106000000001 1670.8353 1673.1592000000001 1675.4824000000001 1677.8049000000001 1680.1268 1682.4480000000001 1684.7683999999999 1687.0880999999999 1689.4073000000001 1691.7257 1694.0435 1696.3605 1698.6768 1700.9924000000001 1703.3074999999999 1705.6216999999999 1707.9353000000001 1710.2483 1712.5605 1714.8721 1717.183 1719.4931999999999 1721.8026 1724.1115 1726.4195999999999 1728.7271000000001 1731.0337999999999 1733.34 1735.6454000000001 1737.9501 1740.2542000000001 1742.5574999999999 1744.8602000000001 1747.1622 1749.4636 1751.7643 1754.0642 1756.3634999999999 1758.6621 1760.9601 1763.2573 1765.5540000000001 1767.8498999999999 1770.1451 1772.4396999999999 1774.7335 1777.0266999999999 1779.3191999999999 1781.6111000000001 1783.9023 1786.1929 1788.4827 1790.7719 1793.0603000000001 1795.3480999999999 1797.6352999999999 1799.9218000000001 1802.2075 1804.4927 1806.7771 1809.0608999999999 1811.3440000000001 1813.6265000000001 1815.9082000000001 1818.1893 1820.4697000000001 1822.7494999999999 1825.0286000000001 1827.307 1829.5847000000001 1831.8617999999999 1834.1382000000001 1836.4139 1838.6890000000001 1840.9634000000001 1843.2372 1845.5102999999999 1847.7826 1850.0544 1852.3253999999999 1854.5958000000001 1856.8656000000001 1859.1346000000001 1861.4031 1863.6709000000001 1865.9380000000001 1868.2043000000001 1870.4701 1872.7352000000001 1874.9996000000001 1877.2634 1879.5264999999999 1881.7889 1884.0508 1886.3118999999999 1888.5724 1890.8322000000001 1893.0913 1895.3497 1897.6075000000001 1899.8647000000001 1902.1212 1904.3770999999999 1906.6322 1908.8867 1911.1405999999999 1913.3938000000001 1915.6464000000001 1917.8982000000001 1920.1495 1922.4 1924.65 1926.8992000000001 1929.1478 1931.3958 1933.6431 1935.8896 1938.1357 1940.3810000000001 1942.6257000000001 1944.8697999999999 1947.1130000000001 1949.3557000000001 1951.5978 1953.8391999999999 1956.08 1958.3200999999999 1960.5596 1962.7982999999999 1965.0364999999999 1967.2738999999999 1969.5107 1971.7469000000001 1973.9825000000001 1976.2174 1978.4517000000001 1980.6851999999999 1982.9182000000001 1985.1505 1987.3821 1989.6130000000001 1991.8434 1994.0731000000001 1996.3021000000001 1998.5306 2000.7583 2002.9855 2005.2119 2007.4376999999999 2009.6628000000001 2011.8875 2014.1113 2016.3344999999999 2018.5571 2020.7791 2023.0003999999999 2025.2209 2027.441 2029.6604 2031.8792000000001 2034.0971999999999 2036.3145999999999 2038.5314000000001 2040.7475999999999 2042.963 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