# Fileset

[Supporting information-Wang-2025_Differential intracellular influence of cancer cells.pdf](https://mdr.nims.go.jp/filesets/277c566a-567b-426e-b624-c62f1a749589/download)

## Creator

Man Wang, Rui Sun, [Huajian Chen](https://orcid.org/0000-0003-1643-9782), [Toru Yoshitomi](https://orcid.org/0000-0003-3847-1812), [Hiroaki Mamiya](https://orcid.org/0000-0002-7840-3008), [Masaki Takeguchi](https://orcid.org/0000-0002-0282-6020), [Naoki Kawazoe](https://orcid.org/0000-0003-3916-0709), Yingnan Yang, [Guoping Chen](https://orcid.org/0000-0001-6753-3678)

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[Differential intracellular influence of cancer cells and normal cells on magnetothermal properties and magnetic hyperthermal effects of magnetic nanoparticles](https://mdr.nims.go.jp/datasets/63ffa3b9-057e-451a-8ad6-e4902b752cea)

## Fulltext

1Supporting Information Differential intracellular influence of cancer cells and normal cells on magnetothermal properties and magnetic hyperthermal effects of magnetic nanoparticlesMan Wang,ab Rui Sun,ab Huajian Chen,a Toru Yoshitomi,a Hiroaki Mamiya,c Masaki Takeguchi,d Naoki Kawazoe,a Yingnan Yange and Guoping Chen*ab a Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Ibaraki 305-0044, Japan. b Graduate School of Science and Technology, University of Tsukuba, Ibaraki 305-8577, Japan.c Research Center for Magnetic and Spintronic Materials, National Institute for Materials Science, Ibaraki 305-0047, Japan.d Research Center for Energy and Environmental Materials, National Institute for Materials Science, Ibaraki 305-0047, Japan.e Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan.*Correspondence: Prof./Dr. Guoping Chen, Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Ibaraki 305-0044, Japan; Graduate School of Science and Technology, University of Tsukuba, Ibaraki 305-8577, Japan. E-mail: Guoping.CHEN@nims.go.jp; ORCID: 0000-0001-6753-3678Supplementary Information (SI) for Materials Horizons.This journal is © The Royal Society of Chemistry 20252Supplementary Figure 1. Characterization of citrate-MNPs. XPS analysis and fitted curves of (A) Fe 2p peaks, (B) O 1s peaks and (C) C 1s peaks of bare-MNPs. (D) FT-IR spectra of bare MNPs, citrate-MNPs and citrate. (E) Normalized field-dependent magnetization curve (M-H) of citrate-MNPs at 300 K. (F) Gross appearance of citrate-MNPs in aqueous solution. Hydrodynamic size distribution of citrate-MNPs in (G) MSCGM medium, (H) OBM medium and (I) DMEM medium. 3Supplementary Figure 2. Viability of MSCs, NHOst cells and MG63 cells after culture with MNPs. Live/dead staining and WST-1 assay of (A, B) MSCs, (C, D) NHOst cells and (E, F) MG63 cells after culture with 0, 30, 60, 120 and 240 μg/mL MNPs for 24 h. Data are expressed as the mean ± S.D. (n = 3). Supplementary Figure 3. Morphology of MSCs, NHOst cells and MG63 cells before and after culture with 120 μg/mL MNPs for 12 h.4Supplementary Figure 4. Cell cytoskeleton assembly of MSCs, NHOst cells and MG63 cells. F-actin staining images of (A) cells cultured without MNPs, (B) cells cultured with MNPs, (C) cells cultured without MNPs but treated Cyto-D (D) cells cultured with MNPs and treated with Cyto-D. Prussian blue staining of internalized MNPs (E) before and (F) after Cyto D treatment.5Supplementary Figure 5. Magnetothermal conversion of MNPs in different cells without any treatment or after Cyto D, fixation and lysis treatments. (A, D, G) Temperature-time curve during 10 min AMF irradiation, (B, E, H) temperature increment after 10 min of AMF irradiation and (C, F, I) SAR of NMPs in (A-C) MSCs, (D-F) NHOst cells and (G-I) MG63 without any treatment or after Cyto D, fixation and lysis treatment.6Supplementary Figure 6. Flow Cytometry analysis of cell apoptosis and apoptosis rate of (A, B) MSCs, (C, D) NHOst cells and (E, F) MG63 cells without MNPs and AMF irradiation (control), without MNPs but with AMF irradiation (AMF), after culture with MNPs before AMF irradiation (MNPs), after culture with MNPs and Cyto D treatment (Cyto D), after culture with MNPs and AMF irradiation (MNPs + AMF), and after culture with MNPs, Cyto D treatment and AMF irradiation (MNPs + AMF + Cyto D).Supplementary Figure 7. AC susceptibility of MNPs within (A) MSCs, (B) NHOst cells and (C) MG63 cells without any treatment or after Cyto D, fixation and lysis treatments. 7Supplementary Table 1. Preparation condition of PEG solutions containing MNPs.MNPs-PEG solutions 10% 20% 25% 30% 40%50% PEG 35000 200 μL 400 μL 500 μL 600 μL 800 μLMilli-Q water 675 μL 475 μL 375 μL 275 μL 75 μL20 mg/mL MNPs 125 μL 125 μL 125 μL 125 μL 125 μL Supplementary Table 2. Preparation condition of PAAm hydrogel embedded with MNPs. MNPs-PAMm hydrogel8%-Bis 0.008%8%-Bis 0.025%8%-Bis 0.03%8%-Bis 0.06%8%-Bis 0.08%20 mg/mL MNPs 125 μL 125 μL 125 μL 125 μL 125 μL64%AAm 125 μL 125 μL 125 μL 125 μL 125 μLMilli-Q water 721 μL 704 μL 699 μL 669 μL 649 μL1%Bis 8 μL 25 μL 30 μL 60 μL 80 μL TEMED 1 μL 1 μL 1 μL 1 μL 1 μLAPS (10%) 20 μL 20 μL 20 μL 20 μL 20 μL