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International Center for Materials Nanoarchitectonics (WPI-MANA)

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[[Research Highlights Vol.18] Intracellular imaging gets interactive](https://mdr.nims.go.jp/datasets/3b723af4-0373-471d-ade9-8f8a3f08b614)

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2022/04/04 10:21 Intracellular imaging gets interactive | MANAhttps://www.nims.go.jp/mana/research/highlights/vol18.html 1/2Previous  Index  NextResearch Highlights[Vol. 18]Intracellular imaging gets interactive29 Aug, 2014A so-called bioparallel chemistry approach is successfully used to image and activate anessential metabolism compound inside a cell.Reduction of ubiquinone-rhodol by NADPH/NADH.A system of compounds has been developed that can signal the presence of certain chemicalcompounds critical to cellular energy metabolism, as well as activating them. The workdemonstrates an approach that the authors suggest could be used to control or investigate cells.They describe their approach as ‘bioparallel chemistry’, emphasising the contrast with widelyknown bioorthogonal chemical reactions that do not interfere with biological processes native tothe cell.The researchers, a collaboration of researchers at Keio University and the International Centre forMaterials Nanoarchitectonics at the National Institute for Materials Science, imaged nicotinamideadenine dinucleotide derivatives – NADH and NADPH. The phosphorylated form, NADPH, is used inphotosynthesis and NADH has been identified as a potential treatment for Alzheimer’s andParkinson’s diseases. The fluorescent compound developed to image NADH and NADPH was amolecule combining ubiquinone – an aromatic compound that has been shown to react NADH andNADPH in vitro – and the fluorophore rhodol.The researchers incubated ubiquinone-rhodol with HeLa cells. When the metabolic process thatgenerates NADH was triggered in the cells the fluorescence was found to decrease as theubiquinone-rhodol was reduced, indicating that NADH increases in cells can be monitored withubiquinone-rhodol.Similarly the fluorescence decreased when an artificial iridium based ‘promoter’ complex wasinjected into the cell, followed by leaching of the cell contents and ultimately death of the cell. Thehttps://www.nims.go.jp/mana/research/highlights/vol17.htmlhttps://www.nims.go.jp/mana/research/highlights/index.htmlhttps://www.nims.go.jp/mana/research/highlights/vol19.html2022/04/04 10:21 Intracellular imaging gets interactive | MANAhttps://www.nims.go.jp/mana/research/highlights/vol18.html 2/2researchers suggest the damage to the cell subsequent to the injection of the artificial promoterwas the result of NADH/NADPH activation upsetting the cell’s homeostasis.“It may be possible to use this strategy to control cellular systems or living systems in general,”suggest the researchers in their conclusion. “This system could form the basis of a new approachin medicine, with the development of novel artificial promoters and reagents.”Reference"Ubiquinone-rhodol (UQ-Rh) for fluorescence imaging of NAD(P)H through intracellular activation"Hirokazu Komatsu, Yutaka Shindo, Kotaro Oka, Jonathan P Hill, and Katsuhiko ArigaJournal : Angew. Chem. Int. EdDOI : 10.1002/anie.201311192AffiliationsInternational Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for MaterialsScience (NIMS), Namiki 1-1, Tsukuba, Ibaraki 305-0044, JapanContact informationInternational Center for Materials Nanoarchitectonics(WPI-MANA)National Institute for Materials Science1-1 Namiki, Tsukuba, Ibaraki 305-0044 JapanPhone: +81-29-860-4710E-mail: mana-pr[AT]ml.nims.go.jphttps://samurai.nims.go.jp/profiles/jonathan_hill?locale=enhttps://samurai.nims.go.jp/profiles/ariga_katsuhiko?locale=enhttps://onlinelibrary.wiley.com/doi/full/10.1002/anie.201311192