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[[Vol. 83]Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier_ MANA.pdf](https://mdr.nims.go.jp/filesets/0b7b45ee-eabf-44cf-90d2-32781a3eb1e0/download)

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International Center for Materials Nanoarchitectonics (WPI-MANA)

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[[Research Highlights Vol.83]Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier](https://mdr.nims.go.jp/datasets/6546668c-4191-4534-b9b2-671968ce29bb)

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Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier| MANAPrevious Index NextResearch Highlights[Vol. 83]Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier3 Aug, 2023Scientists at the Research Center for Materials Nanoarchitectonics (MANA) have developedmicrometer-sized "liquid marbles" that can encapsulate single to several living cells, therebyaiding single-cell studies.CG Graphic：Mizuki Tenjimbayashi, Independent ScientistTo analyze individual cells, the desired number of cells must be isolated spatially. This often requiresextensive training and use of expensive laboratory equipment. MANA scientists have recently overcomethis hurdle using nanotechnology. They coated cell-suspension microdroplets with superhydrophobicnanoparticles -- tiny particles that possess surfaces with exceptional water-repellent properties -- andcreated spherical structures known as "drycells," with customizable sizes ranging from one to severalhundred micrometers, which can be handled and picked up with ease. This will make cell picking easierand greatly facilitate single-cell analysis.To achieve this, the research team hand-sprayed suspended cells to create a cellular "mist." UponResearchQuantum Materials FieldNanomaterials FieldResearch SupportResearch HighlightsHot TopicsHome  > Research  > Research Highlights  > Vol. 83 Novel “Drycell” Microdroplets ･･･About MANA Research People News Room Outreach Employment AlumniSite Map Contact Us Access to MANA Website System Requirements   Text size  Standard Large  Japanese Page2024/07/16 13:37 Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier| MANAhttps://www.nims.go.jp/mana/research/highlights/vol83.html 1/3https://www.nims.go.jp/mana/research/highlights/vol82.htmlhttps://www.nims.go.jp/mana/research/highlights/index.htmlhttps://www.nims.go.jp/mana/research/highlights/vol84.htmlhttps://samurai.nims.go.jp/profiles/tenjimbayashi_mizuki?locale=enhttps://www.nims.go.jp/mana/research/index.htmlhttps://www.nims.go.jp/mana/research/quantum_material.htmlhttps://www.nims.go.jp/mana/research/nano_material.htmlhttps://www.nims.go.jp/mana/research/researcher_support.htmlhttps://www.nims.go.jp/mana/research/highlights/index.htmlhttps://www.nims.go.jp/mana/research/hottopics/index.htmlhttps://www.nims.go.jp/mana/jp/index.htmlhttps://www.nims.go.jp/mana/research/index.htmlhttps://www.nims.go.jp/mana/research/highlights/index.htmlhttp://www.jsps.go.jp/english/e-toplevel/http://www.jsps.go.jp/english/e-toplevel/https://www.nims.go.jp/mana/index.htmlhttps://www.nims.go.jp/mana/index.htmlhttps://www.nims.go.jp/mana/about/index.htmlhttps://www.nims.go.jp/mana/research/index.htmlhttps://www.nims.go.jp/mana/member/index.htmlhttps://www.nims.go.jp/mana/news_room/2024.htmlhttps://www.nims.go.jp/mana/pror/index.htmlhttps://www.nims.go.jp/mana/recruit/index.htmlhttps://www.nims.go.jp/mana/alumni/index.htmlhttps://www.nims.go.jp/mana/siteinfo/sitemap.htmlhttps://www.nims.go.jp/mana/siteinfo/inquiry.htmlhttps://www.nims.go.jp/mana/siteinfo/access.htmlhttps://www.nims.go.jp/mana/siteinfo/accessibility.htmlhttps://www.nims.go.jp/mana/jp/research/highlights/vol83.htmlcoming into contact with a vibrating nanoparticle bed, the cell-containing mist droplets instantly gotcoated with superhydrophobic-fumed silica nanoparticles, thus producing liquid marble-like drycells witha dry powder-like outer layer surrounding an inner liquid core. In fact, the scientists could create drycellswith >95% inner aqueous content surrounded by a dry outer layer. These droplets exhibited powder-likesmooth flow and could easily be picked up with a pair of tweezers without suffering any damage orspilling the inner liquid. Moreover, the superhydrophobic silica nanoparticles in the surface coatingprevented the droplets from merging.The scientists could sort the drycells according to size through sieving. They were also able tosimultaneously encapsulate normal and cancerous cells as well as creating several cell colonies insidethese drycells. Further, they could introduce liquids into, as well as withdraw, the liquid from thesedroplets with ease. Finally, they observed that centrifugation could separate the nanoparticles from thecell-suspension layers, which makes the process recyclable."Our method of drycell production efficiently facilitates cell picking and lowers the barrier for single-cellstudies and will greatly improve the accessibility and productivity of single-cell analysis," concludes Dr.Koichiro Uto, speaking on behalf of study co-authors Mizuki Tenjimbayashi and Shota Yamamoto.Reference“Drycells: Cell-Suspension Micro Liquid Marbles for Single-Cell Picking”Mizuki Tenjimbayashi, Shota Yamamoto, and Koichiro UtoJournal: Advanced Materials 2023 (July 27, 2023)DOI : 10.1002/adma.202300486AffiliationsResearch Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science(NIMS), Namiki 1-1, Tsukuba, Ibaraki 305-0044, JapanResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS),Namiki 1-1, Tsukuba, Ibaraki 305-0044, JapanReference : Mizuki Tenjimbayashi, Independent Scientist, and his colleagues' researchis selected for the cover art of the journal "Advanced Materials"Contact informationResearch Center for Materials Nanoarchitectonics (MANA)National Institute for Materials Science1-1 Namiki, Tsukuba, Ibaraki 305-0044 JapanPhone: +81-29-860-4710E-mail: mana-pr[AT]nims.go.jpTo receive our e-mail newsletter “MANA Research Highlights”, please send an e-mail with "MANA ResearchHighlights request” in the subject line or main text to the following address: mana-pr_at_nims.go.jp *Pleasechange "_at_ " in the email address to @.Page Top2024/07/16 13:37 Novel “Drycell” Microdroplets to Make Handling a Single Cell Easier| MANAhttps://www.nims.go.jp/mana/research/highlights/vol83.html 2/3https://samurai.nims.go.jp/profiles/tenjimbayashi_mizuki?locale=enhttps://doi.org/10.1002/adma.202300486https://www.nims.go.jp/eng/research/mana/index.htmlhttps://www.nims.go.jp/eng/research/macromolecules-biomaterials/index.htmlhttps://www.nims.go.jp/mana/news_room/news/2023072801.htmlhttps://www.nims.go.jp/mana/news_room/news/2023072801.htmlResearch Center for Materials Nanoarchitectonics (MANA)National Institute for Materials Science (NIMS)1-1 Namiki Tsukuba, Ibaraki 305-0044 JAPAN+81-29-860-4709E-mail: mana[AT]nims.go.jpCopyright © National Institute for Materials Science. 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