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[[Vol. 3]Bone tissue engineering_ Attaching proteins for better regeneration_ WPI-MANA.pdf](https://mdr.nims.go.jp/filesets/08cb47c4-337c-4baf-a568-80e8d59a20c6/download)

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International Center for Materials Nanoarchitectonics (WPI-MANA)

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[[Research Highlights Vol.3] Bone tissue engineering: Attaching proteins for better regeneration](https://mdr.nims.go.jp/datasets/062f3f88-fc92-44c6-8e40-a1acdd207632)

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2022/04/04 10:30 Bone tissue engineering: Attaching proteins for better regeneration| MANAhttps://www.nims.go.jp/mana/research/highlights/vol3.html 1/2Previous  Index  NextResearch Highlights[Vol. 3]Bone tissue engineering: Attaching proteins for better regeneration26 Jul, 2012Researchers in Japan demonstrate a new protein binding approach foreffectively promoting bone regeneration. Current treatments for bone defectsand bone tissue regeneration have significant limitations. Now a new methodthat immobilizes a fusion protein in a hybrid collagen-polymer supportivescaffold shows promise for bone tissue engineering.Guoping Chen, and Naoki Kawazoe at the Tissue Regeneration Materials Unit, International Centerfor Materials Nanoarchitectonics (MANA), National Institute for Materials Science, and researchersat RIKEN, the Immuno-Biological Laboratories Co., Ltd, and the National Institute for Child Healthand Development added a collagen-binding domain from fibronectin to BMP4, a protein thatpromotes bone regeneration. The new fusion protein, BMP4-CBD, was immobilized in a hybridscaffold of natural collagen sponge and a strong synthetic polymer, poly(lactic-co-glycolic acid)(PLGA). The hybrid scaffold provides space and support for the bone cell growth.The researchers investigated the effect of BMP4-CBD immobilized in the collagen-PLGA scaffold byin vivo implantationfor four weeks. They compared the approach with a range of controls includingscaffolds with wild-type BMP4 without the collagen-binding domain and scaffolds with just thecollagen-binding domain. The expression of specific osteogenetic markers used as indicators ofhttps://www.nims.go.jp/mana/research/highlights/vol2.htmlhttps://www.nims.go.jp/mana/research/highlights/index.htmlhttps://www.nims.go.jp/mana/research/highlights/vol4.html2022/04/04 10:30 Bone tissue engineering: Attaching proteins for better regeneration| MANAhttps://www.nims.go.jp/mana/research/highlights/vol3.html 2/2bone tissue regeneration was much higher for BMP4-CBD-immobilized hybrid scaffolds. UsingBMP4-CBD also promoted calcification."These effects should be attributed to the retention of more effective molecules due to the specificbinding of the fusion BMP4 to the collagen," say the authors. The stimulation effect of the proteinpromoting the bone regeneration is thus maintained over a longer period. The research maybenefit patients suffering from bone defects in the future.Reference"Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold forenhanced osteoinductivity"Hongxu Lu, Naoki Kawazoe, Takashi Kitajima, Yuka Myoken, Masahiro Tomita, Akihiro Umezawa,Guoping Chen, and Yoshihiro ItoJournal: Biomaterials. 33, 6140–6146 (2011) [26 September 2012]DOI : 10.1016/j.biomaterials.2012.05.038AffiliationsInternational Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for MaterialsScience (NIMS), Namiki 1-1, Tsukuba, Ibaraki 305-0044, JapanContact informationInternational Center for Materials Nanoarchitectonics(WPI-MANA)National Institute for Materials Science1-1 Namiki, Tsukuba, Ibaraki 305-0044 JapanPhone: +81-29-860-4710E-mail: mana-pr[AT]ml.nims.go.jphttps://doi.org/10.1016/j.biomaterials.2012.05.038