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We have previously reported that a novel bioresorbable self-setting injectable bone paste composed of hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) and (3-glycidoxypropyl)trimethoxysilane (GPTMS) was successfully prepared and was replaced with new bone within 3 months of implantation in defects created in porcine tibia. In this study, the HAp/Col-GPTMS paste was implanted into bone defects in rat tibiae to investigate the initial kinetics and bone tissue response. Even though more than 35% of GPTMS molecules should be eluted rapidly from directly injected pastes according to previously reported cell culture tests, in this study, energy dispersive X-ray spectrometry did not detect Si (GPTMS) deposition in tissues surrounding the paste at one day post-implantation. Further, no abnormal inflammatory responses were observed in the surrounding tissues over the test period for both directly injected and pre-hardened pastes. Companying these observations with the results of the previous animal test (in which the paste was fully resorbed and was substituted with new bone), the eluted GPTMS resolved in no harm in vivo from the initial to final (completely resorbed) stages. Material resorption rates calculated from X-ray micro-computed tomography (µ-CT) images decreased with increasing in GPTMS concentration. Histological observations indicated that tartrate-resistant acid phosphatase (TRAP) active cells, (assumed to be osteoclasts), exist on the periphery of pastes. This result suggested that the paste was resorbed by osteoclasts in the same way as the HAp/Col. Since a good correlation was observed between TRAP active areas in histological sections and material resorption rate calculated from µ-CT, the TRAP activity coverage ratio offers the possibility to estimate the osteoclastic resorption ratio of materials which are replaced with bone via bone remodeling process.

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• Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International
  This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Keyword: (3-glycidoxypropyl)trimethoxysilane, bioresorbability evaluation, bone tissue reaction, hydroxyapatite/collagen, injectable bone paste

Date published: 2024-07-25

Publisher: Wiley

Journal:

• Journal of Biomedical Materials Research Part B: Applied Biomaterials (ISSN: 15524973) vol. 112 issue. 8 e35451

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Manuscript type: Publisher's version (Version of record)

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First published URL: https://doi.org/10.1002/jbm.b.35451

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Updated at: 2024-09-02 16:30:11 +0900

Published on MDR: 2024-09-02 16:30:11 +0900