# Influence of viscosity on bone marrow-derived mesenchymal stem cells trilineage differentiation during 3D culture

https://mdr.nims.go.jp/datasets/f9abb2a6-8841-4f75-9b7c-cef692867e59

## File

- [Chengyu Lu_author submitted manuscript-draft.pdf](https://mdr.nims.go.jp/filesets/7adaa9b5-ab91-43fb-a694-a1e42518d89a/download) ([Detail](https://mdr.nims.go.jp/filesets/7adaa9b5-ab91-43fb-a694-a1e42518d89a.md))

## Id

f9abb2a6-8841-4f75-9b7c-cef692867e59

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-10-13T00:50:39.816336Z

## Updated at

2025-10-14T08:11:30.332507Z

## Published at

2026-09-22T23:33:55.025923Z

## Doi

https://doi.org/10.48505/nims.5807

## First published url

https://doi.org/10.1088/1758-5090/ae0661

## Date published

2025-10-01

## Recorded date published

2025-10-1

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Influence of viscosity on bone marrow-derived mesenchymal stem cells trilineage
    differentiation during 3D culture
  title_type: original
  lang: en

## Description

- description: In this study, the influence of viscosity on trilineage differentiation
    (adipogenesis, chondrogenesis and osteogenesis) of human bone marrow-derived mesenchymal
    stem cells (hMSCs) was disclosed by three-dimensionally culturing hMSCs in viscous
    media. The viscosity was modulated using bioinert polyethylene glycol (PEG) at
    a range of 88.8 to 645.5 cP. A cuboid agarose hydrogel container was used to encapsulate
    the cells and viscous media to prevent cell leakage and PEG diffusion during cell
    culture. Viscosity showed inhibitory effects on trilineage differentiation of
    hMSCs during 3D culture in viscous media containing PEG. The inhibitory effect
    on adipogenic and chondrogenic differentiation was stronger than that on osteogenic
    differentiation. Viscosity strongly promoted cell proliferation during chondrogenesis,
    and weakly promoted cell proliferation during osteogenesis, while inhibited cell
    proliferation during adipogenesis.
  description_type: abstract
  lang: und

## Creator

- name: Chengyu Lu
  role: author
  orcid: https://orcid.org/0000-0002-8334-3177
- name: Jing Zheng
  role: author
- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
- name: Man Wang
  role: author
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: IOP Publishing

## Managing organization



## Keyword

- subject: viscosity
  schema: not_defined
- subject: 3D culture
  schema: not_defined
- subject: adipogenic differentiation
  schema: not_defined
- subject: chondrogenic differentiation
  schema: not_defined
- subject: osteogenic differentiation
  schema: not_defined
- subject: mesenchymal stem cell
  schema: not_defined

## Rights

- description: This is the Accepted Manuscript version of an article accepted for
    publication in Biofabrication. IOP Publishing Ltd is not responsible for any errors
    or omissions in this version of the manuscript or any version derived from it.  The
    Version of Record is available online at 10.1088/1758-5090/ae0661.
  identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2025-09-23
end_date: 2026-09-23

## Journal

- title: Biofabrication
  issn: '17585082'
  volume: '17'
  issue: '4'
  article_number: '45016'

## Conference



## Related item



## Funding

- identifier: 21H03830
  funder_name: Japan Society for the Promotion of Science

## Instrument



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## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



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## Fileset

- id: 7adaa9b5-ab91-43fb-a694-a1e42518d89a
  filename: Chengyu Lu_author submitted manuscript-draft.pdf
  content_type: application/pdf
  size: 1487428
  md5: c9212224586e2174f33f8e807884290f

## Thumbnail

fileset_id: 7adaa9b5-ab91-43fb-a694-a1e42518d89a
filename: Chengyu Lu_author submitted manuscript-draft.pdf