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In this study, the influence of viscosity on trilineage differentiation (adipogenesis, chondrogenesis and osteogenesis) of human bone marrow-derived mesenchymal stem cells (hMSCs) was disclosed by three-dimensionally culturing hMSCs in viscous media. The viscosity was modulated using bioinert polyethylene glycol (PEG) at a range of 88.8 to 645.5 cP. A cuboid agarose hydrogel container was used to encapsulate the cells and viscous media to prevent cell leakage and PEG diffusion during cell culture. Viscosity showed inhibitory effects on trilineage differentiation of hMSCs during 3D culture in viscous media containing PEG. The inhibitory effect on adipogenic and chondrogenic differentiation was stronger than that on osteogenic differentiation. Viscosity strongly promoted cell proliferation during chondrogenesis, and weakly promoted cell proliferation during osteogenesis, while inhibited cell proliferation during adipogenesis.

権利情報:

• Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International
  

  This is the Accepted Manuscript version of an article accepted for publication in Biofabrication. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at 10.1088/1758-5090/ae0661.

キーワード: viscosity, 3D culture, adipogenic differentiation, chondrogenic differentiation, osteogenic differentiation, mesenchymal stem cell

刊行年月日: 2025-10-01

出版者: IOP Publishing

掲載誌:

• Biofabrication (ISSN: 17585082) vol. 17 issue. 4 45016

研究助成金:

• Japan Society for the Promotion of Science 21H03830

原稿種別: 著者最終稿 (Accepted manuscript)

MDR DOI: https://doi.org/10.48505/nims.5807

公開URL: https://doi.org/10.1088/1758-5090/ae0661

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更新時刻: 2025-10-14 17:11:30 +0900

MDRでの公開時刻: 2026-09-23 08:33:55 +0900