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説明:

Quantitative investigation of protein adsorption and desorption at triacylglycerol/water interfaces is of great importance for understanding the properties and functions of intracellular lipid droplets. In this study, we investigated the adsorption and desorption of cytochrome c, lysozyme, and bovine serum albumin at the tricaprylin/water interface covered with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) using a surface pressure measurement system and a pendant drop tensiometer. The aim of this study was to understand the quantitative relationships between the area per DOPC molecule and the protein adsorption and desorption. We found that the area per DOPC molecule required to preclude bovine serum albumin adsorption was much smaller than that required to preclude the adsorption of cytochrome c or lysozyme. However, the area per DOPC molecule required for all of the bound protein molecules to desorb from the interface was independent of the type of protein, and this threshold area per DOPC molecule was in good agreement with an area per lipid value previously reported for fully hydrated DOPC bilayers.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Lipid droplets, triacylglycerol, oil/water interface, protein

刊行年月日: 2025-09-23

出版者: American Chemical Society (ACS)

掲載誌:

• Langmuir (ISSN: 07437463) vol. 41 issue. 37 p. 25431-25438

研究助成金:

• Japan Society for the Promotion of Science 22K05179

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1021/acs.langmuir.5c03216

関連資料:

• is_supplemented_by
• Quantifying the Thresholds of the Phospholipid Surface Density for Nonspecific Protein Adsorption and Desorption at the Triacylglycerol/Water Interface (dataset)
• https://doi.org/10.48505/nims.5776

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更新時刻: 2025-09-24 16:30:20 +0900

MDRでの公開時刻: 2025-09-24 16:19:15 +0900