# Enrichment of horizontally transferred gene clusters in bacterial extracellular vesicles via non lytic mechanisms

https://mdr.nims.go.jp/datasets/eaf84ac0-5f6f-4a1d-924d-ac00039e1011

## File

- [Takano Sotaro ISMEj 2025.pdf](https://mdr.nims.go.jp/filesets/e102a355-6658-405f-8ae3-578d30deb7f5/download) ([Detail](https://mdr.nims.go.jp/filesets/e102a355-6658-405f-8ae3-578d30deb7f5.md))
- [supplemental_materials_20250825plain_wraf193_1.pdf](https://mdr.nims.go.jp/filesets/acec2f47-48ee-46cc-bf98-8c8dc533b91d/download) ([Detail](https://mdr.nims.go.jp/filesets/acec2f47-48ee-46cc-bf98-8c8dc533b91d.md))
- [supplementary_data_wraf193.xlsx](https://mdr.nims.go.jp/filesets/d377f34e-ad55-4df1-bdd6-b386fb098b02/download) ([Detail](https://mdr.nims.go.jp/filesets/d377f34e-ad55-4df1-bdd6-b386fb098b02.md))

## Id

eaf84ac0-5f6f-4a1d-924d-ac00039e1011

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-01-22T00:11:19.660766Z

## Updated at

2026-01-22T00:56:49.624376Z

## Published at

2026-01-22T03:26:36.358937Z

## Doi



## First published url

https://doi.org/10.1093/ismejo/wraf193

## Date published

2025-01-02

## Recorded date published

2025-1-2

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Enrichment of horizontally transferred gene clusters in bacterial extracellular
    vesicles via non lytic mechanisms
  title_type: original
  lang: en

## Description

- description: 'Bacterial extracellular vesicles are emerging as key mediators of
    horizontal gene transfer, enhancing microbial adaptability. A critical factor
    determining the effectiveness of horizontal gene transfer is the fraction of vesicles
    containing specific functional genes. However, the proportion of containing specific
    DNA fragments has not been adequately determined, which hinders the understanding
    of the conditions and mechanisms that facilitate the incorporation of specific
    genes into the vesicles and possible evolutionary roles of vesicle-derived DNA.
    Here, we demonstrate that enrichment of horizontally transferred genes into bacterial
    extracellular vesicles is driven by cellular processes by profiling the DNA content
    of hundreds of individual vesicles using a microdroplet-based sequencing technique. '
  description_type: abstract
  lang: und

## Creator

- name: Sotaro Takano
  role: author
  orcid: https://orcid.org/0000-0003-2858-6587
- name: Satoshi Takenawa
  role: author
- name: Divya Naradasu
  role: author
- name: Kangmin Yan
  role: author
- name: Xinxin Wen
  role: author
- name: Tomoko Maehara
  role: author
- name: Nobuhiko Nomura
  role: author
- name: Nozomu Obana
  role: author
- name: Masanori Toyofuku
  role: author
- name: Michihiko Usui
  role: author
- name: Wataru Ariyoshi
  role: author
- name: Akihiro Okamoto
  role: author
  orcid: https://orcid.org/0000-0002-8102-4316

## Contact agent



## Publisher

organization: Oxford University Press (OUP)

## Managing organization



## Keyword

- subject: membrane vesicle
  schema: not_defined
- subject: horizontal gene transfer
  schema: not_defined
- subject: oral microbiota
  schema: not_defined
- subject: CRISPR-Cas
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: The ISME Journal
  issn: '17517362'
  volume: '19'
  issue: '1'
  article_number: wraf193

## Conference



## Related item



## Funding

- identifier: JPMJPR19H1
  funder_name: JST
- identifier: '24028593'
  funder_name: JST
- identifier: 22H02265
  funder_name: JSPS
- identifier: 24K17823
  funder_name: JSPS
- identifier: 19gm6010002h0004
  funder_name: The Japan Agency for Medical Research and Development
- identifier: 21ae0121044h0001
  funder_name: The Japan Agency for Medical Research and Development
- identifier: PMXP1224NM5397
  funder_name: Advanced Research Infrastructure for Materials and Nanotechnology in
    Japan of the Ministry of Education, Culture, Sports, Science and Technology (MEXT)

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: e102a355-6658-405f-8ae3-578d30deb7f5
  filename: Takano Sotaro ISMEj 2025.pdf
  content_type: application/pdf
  size: 2079093
  md5: 10a089b99c2e5eed2c36cdc8e6a8c892
- id: acec2f47-48ee-46cc-bf98-8c8dc533b91d
  filename: supplemental_materials_20250825plain_wraf193_1.pdf
  content_type: application/pdf
  size: 5186297
  md5: 9253797818d1e7e582517a67d0e1c331
- id: d377f34e-ad55-4df1-bdd6-b386fb098b02
  filename: supplementary_data_wraf193.xlsx
  content_type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
  size: 732959
  md5: d565f7f60594b27ddf758abba1b3b559

## Thumbnail

fileset_id: e102a355-6658-405f-8ae3-578d30deb7f5
filename: Takano Sotaro ISMEj 2025.pdf