# Immunostimulatory Effects of Guanine-Quadruplex Topologies as Scaffolds for CpG Oligodeoxynucleotides

https://mdr.nims.go.jp/datasets/ea9b2ba4-40f2-45fb-a1ad-191c440735df

## File

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- [Immunostimulatory Effects of Guani-2025-Patha1.pdf](https://mdr.nims.go.jp/filesets/1e29f602-289a-4fec-991e-a0d8e5a903e9/download) ([Detail](https://mdr.nims.go.jp/filesets/1e29f602-289a-4fec-991e-a0d8e5a903e9.md))

## Id

ea9b2ba4-40f2-45fb-a1ad-191c440735df

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-03-27T01:07:44.166145Z

## Updated at

2025-03-27T08:44:07.106017Z

## Published at

2025-03-27T08:44:07.277489Z

## Doi



## First published url

https://doi.org/10.3390/biom15010095

## Date published

2025-01-10

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Immunostimulatory Effects of Guanine-Quadruplex Topologies as Scaffolds for
    CpG Oligodeoxynucleotides
  title_type: original
  lang: en

## Description

- description: Synthetic cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODNs)
    are promising candidates for vaccine adjuvants, because they activate immune responses
    through the Toll-like receptor 9 (TLR9) pathway. However, unmodified CpG ODNs
    are quickly degraded by serum nucleases, and their negative charge hinders cellular
    uptake, limiting their clinical application. Our group previously reported that
    guanine-quadruplex (G4)-forming CpG ODNs exhibit enhanced stability and cellular
    uptake. G4 structures can form in parallel, anti-parallel, or hybrid topologies,
    depending on strand orientation, but the effects of these topologies on CpG ODNs
    have not yet been explored. In this study, we designed three distinct G4 topologies
    as scaffolds for CpG ODNs. Among the three topology, the parallel G4 CpG ODN demonstrated
    the highest serum stability and cellular uptake, resulting in the strongest immune
    response from macrophage cells. Additionally, we investigated the binding affinities
    of the different G4 topologies to macrophage scavenger receptor-1 and TLR9, both
    of which are key to immune activation. These findings provide valuable insights
    into the development of CpG ODN-based vaccine adjuvants.
  description_type: abstract
  lang: und

## Creator

- name: Soumitra Pathak
  role: author
  orcid: https://orcid.org/0000-0002-1007-4738
- name: Nguyen Bui Thao Le
  role: author
  orcid: https://orcid.org/0000-0002-4797-2339
- name: Taiji Oyama
  role: author
  orcid: https://orcid.org/0000-0003-1403-073X
- name: Yusuke Odahara
  role: author
- name: Atsuya Momotake
  role: author
- name: Kazunori Ikebukuro
  role: author
  orcid: https://orcid.org/0000-0003-2838-0562
- name: Chiho Kataoka-Hamai
  role: author
  orcid: https://orcid.org/0000-0002-4068-0405
- name: Chiaki Yoshikawa
  role: author
  orcid: https://orcid.org/0000-0002-6589-387X
- name: Kohsaku Kawakami
  role: author
  orcid: https://orcid.org/0000-0002-3466-9365
- name: Yoshihisa Kaizuka
  role: author
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: CpG oligodeoxynucleotides
  schema: not_defined
- subject: adjuvants
  schema: not_defined
- subject: guanine-quadruplex
  schema: not_defined
- subject: immune response
  schema: not_defined
- subject: serum stability
  schema: not_defined
- subject: toll like receptor 9
  schema: not_defined
- subject: topology
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biomolecules
  issn: 2218273X
  volume: '15'
  issue: '1'
  article_number: '95'

## Conference



## Related item



## Funding

- identifier: 21K19057
  funder_name: Japan Society for the Promotion of Science KAKENHI
- identifier: TK21-017
  funder_name: TIA collaborative research program “Kakehashi”
- identifier: TK22-033
  funder_name: TIA collaborative research program “Kakehashi”

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

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  filename: Immunostimulatory Effects of Guani-2025-Pathak.pdf
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  size: 2707967
  md5: 0f0d615b5121568f1b970a5f2a43077e
- id: 1e29f602-289a-4fec-991e-a0d8e5a903e9
  filename: Immunostimulatory Effects of Guani-2025-Patha1.pdf
  content_type: application/pdf
  size: 1218520
  md5: a505fab155ee73557b18780d58aaa1f1

## Thumbnail

fileset_id: 1e29f602-289a-4fec-991e-a0d8e5a903e9
filename: Immunostimulatory Effects of Guani-2025-Patha1.pdf