# Nanowire Electrode Structures Enhanced Direct Extracellular Electron Transport via Cell-Surface Multi-Heme Cytochromes in Desulfovibrio ferrophilus IS5 https://mdr.nims.go.jp/datasets/db7c727b-7a0d-48ce-b0e8-3b10efd57d52 ## File - [electrochem-05-00021-v2.pdf](https://mdr.nims.go.jp/filesets/674f59b7-f407-4790-b675-a1ceaee4d892/download) ([Detail](https://mdr.nims.go.jp/filesets/674f59b7-f407-4790-b675-a1ceaee4d892.md)) ## Id db7c727b-7a0d-48ce-b0e8-3b10efd57d52 ## Local identifier ## Visibility open_to_public ## State published ## Created at 2024-09-02T01:47:24.697860Z ## Updated at 2024-09-02T23:30:25.372468Z ## Published at 2024-09-02T23:30:25.470381Z ## Doi ## First published url https://doi.org/10.3390/electrochem5030021 ## Date published 2024-08-13 ## Recorded date published ## Resource type journal_article ## Manuscript type vor ## Collection ## Title - title: Nanowire Electrode Structures Enhanced Direct Extracellular Electron Transport via Cell-Surface Multi-Heme Cytochromes in Desulfovibrio ferrophilus IS5 title_type: original lang: en ## Description - description: "settingsOrder Article Reprints\r\nOpen AccessArticle\r\nNanowire Electrode Structures Enhanced Direct Extracellular Electron Transport via Cell-Surface Multi-Heme Cytochromes in Desulfovibrio ferrophilus IS5\r\nby Xiao Deng 1,*ORCID,Wipakorn Jevasuwan 2ORCID,Naoki Fukata 2ORCID andAkihiro Okamoto 1,3,4,5,*ORCID\r\n1\r\nResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan\r\n2\r\nResearch Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan\r\n3\r\nGraduate School of Chemical Sciences and Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo 060-8628, Japan\r\n4\r\nGraduate School of Science and Engineering, College of Science and Engineering, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8573, Japan\r\n5\r\nLiving Systems Materialogy (LiSM) Research Group, International Research Frontiers Initiative (IRFI), Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan\r\n*\r\nAuthors to whom correspondence should be addressed.\r\nElectrochem 2024, 5(3), 330-340; https://doi.org/10.3390/electrochem5030021\r\nSubmission received: 1 July 2024 / Revised: 31 July 2024 / Accepted: 7 August 2024 / Published: 13 August 2024\r\n(This article belongs to the Collection Feature Papers in Electrochemistry)\r\nDownloadkeyboard_arrow_down Browse Figures Versions Notes\r\nAbstract\r\nExtracellular electron transfer (EET) by sulfate-reducing bacteria (SRB), such as Desulfovibrio ferrophilus IS5, enables bacterial interactions with minerals, which are vital for biogeochemical cycling and environmental chemistry. Here, we explore the direct EET mechanisms through outer-membrane cytochromes (OMCs) using IS5 as a model SRB. We employed nanostructured electrodes arrayed with 0, 50, 200, and 500 nm long nanowires (NWs) coated with indium–tin–doped oxide to examine the impact of electrode morphology on the direct EET efficacy. Compared to flat electrodes, NW electrodes significantly enhanced current production in IS5 with OMCs. However, this enhancement was diminished when OMC expression was reduced. Differential pulse voltammetry revealed that NW electrodes specifically augmented redox peaks associated with OMCs without affecting those related to redox mediators, suggesting that NWs foster direct EET through OMCs. Scanning electron microscopy observations following electrochemical analyses revealed a novel vertical cell attachment and aggregation on NW electrodes, contrasting with the horizontal monolayer cell attachment on flat electrodes. This study presents the first evidence of the critical role of electrode nanoscale topography in modulating SRB cell orientation and aggregation behavior. The findings underscore the significant influence of electrode morphology on the direct EET kinetics, highlighting the potential impact of mineral morphology on mineral reduction and biogeochemical processes." description_type: abstract lang: und ## Creator - name: Xiao Deng role: author orcid: https://orcid.org/0000-0002-9006-2322 organization: National Institute for Materials Science - name: Wipakorn Jevasuwan role: author orcid: https://orcid.org/0000-0001-9117-2497 organization: National Institute for Materials Science - name: Naoki Fukata role: author orcid: https://orcid.org/0000-0002-0986-8485 organization: National Institute for Materials Science - name: Akihiro Okamoto role: author orcid: https://orcid.org/0000-0002-8102-4316 organization: National Institute for Materials Science ## Contact agent ## Publisher organization: MDPI AG ## Managing organization ## Keyword - subject: cell attachment schema: not_defined - subject: differential pulse voltammetry schema: not_defined - subject: electrochemical analysis schema: not_defined - subject: nanostructure schema: not_defined - subject: outer-membrane cytochrome schema: not_defined - subject: scanning electron microscopy schema: not_defined ## Rights - identifier: https://creativecommons.org/licenses/by/4.0/ ## Other identifier(s) ## Data origin ## Embargo ## Journal - title: Electrochem issn: '26733293' volume: '5' issue: '3' start_page: 330 end_page: 340 ## Conference ## Related item ## Funding - funder_name: Murata Science and Education Foundation, Kyoto, Japan - identifier: 22H02265 funder_name: JSPS KAKENHI - identifier: 22KK0242 funder_name: JSPS KAKENHI - identifier: 23K23532 funder_name: JSPS KAKENHI - identifier: JPJA20P20333393 funder_name: JAEA Nuclear Energy S&T and Human Resource Development Project through concentrating wisdom ## Instrument ## Instrument operator ## Instrument managing organization ## Measurement method ## Specimen ## Chemical composition ## Structure for specimen ## Structural feature for specimen ## Specific property for specimen ## Process for specimen treatment ## Computational method ## Energy level/transition state ## Software ## Custom property ## Fileset - id: 674f59b7-f407-4790-b675-a1ceaee4d892 filename: electrochem-05-00021-v2.pdf content_type: application/pdf size: 5709063 md5: 92aa72d3a404c1208b057986870a6522 ## Thumbnail fileset_id: 674f59b7-f407-4790-b675-a1ceaee4d892 filename: electrochem-05-00021-v2.pdf