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Nanowire Electrode Structures Enhanced Direct Extracellular Electron Transport via Cell-Surface Multi-Heme Cytochromes in Desulfovibrio ferrophilus IS5
by Xiao Deng 1,*ORCID,Wipakorn Jevasuwan 2ORCID,Naoki Fukata 2ORCID andAkihiro Okamoto 1,3,4,5,*ORCID
1
Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan
2
Research Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan
3
Graduate School of Chemical Sciences and Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo 060-8628, Japan
4
Graduate School of Science and Engineering, College of Science and Engineering, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8573, Japan
5
Living Systems Materialogy (LiSM) Research Group, International Research Frontiers Initiative (IRFI), Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan
*
Authors to whom correspondence should be addressed.
Electrochem 2024, 5(3), 330-340; https://doi.org/10.3390/electrochem5030021
Submission received: 1 July 2024 / Revised: 31 July 2024 / Accepted: 7 August 2024 / Published: 13 August 2024
(This article belongs to the Collection Feature Papers in Electrochemistry)
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Abstract
Extracellular electron transfer (EET) by sulfate-reducing bacteria (SRB), such as Desulfovibrio ferrophilus IS5, enables bacterial interactions with minerals, which are vital for biogeochemical cycling and environmental chemistry. Here, we explore the direct EET mechanisms through outer-membrane cytochromes (OMCs) using IS5 as a model SRB. We employed nanostructured electrodes arrayed with 0, 50, 200, and 500 nm long nanowires (NWs) coated with indium–tin–doped oxide to examine the impact of electrode morphology on the direct EET efficacy. Compared to flat electrodes, NW electrodes significantly enhanced current production in IS5 with OMCs. However, this enhancement was diminished when OMC expression was reduced. Differential pulse voltammetry revealed that NW electrodes specifically augmented redox peaks associated with OMCs without affecting those related to redox mediators, suggesting that NWs foster direct EET through OMCs. Scanning electron microscopy observations following electrochemical analyses revealed a novel vertical cell attachment and aggregation on NW electrodes, contrasting with the horizontal monolayer cell attachment on flat electrodes. This study presents the first evidence of the critical role of electrode nanoscale topography in modulating SRB cell orientation and aggregation behavior. The findings underscore the significant influence of electrode morphology on the direct EET kinetics, highlighting the potential impact of mineral morphology on mineral reduction and biogeochemical processes.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: cell attachment, differential pulse voltammetry, electrochemical analysis, nanostructure, outer-membrane cytochrome, scanning electron microscopy

刊行年月日: 2024-08-13

出版者: MDPI AG

掲載誌:

• Electrochem (ISSN: 26733293) vol. 5 issue. 3 p. 330-340

研究助成金:

• Murata Science and Education Foundation, Kyoto, Japan
• JSPS KAKENHI 22H02265
• JSPS KAKENHI 22KK0242
• JSPS KAKENHI 23K23532
• JAEA Nuclear Energy S&T and Human Resource Development Project through concentrating wisdom JPJA20P20333393

原稿種別: 出版者版 (Version of record)

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公開URL: https://doi.org/10.3390/electrochem5030021

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更新時刻: 2024-09-03 08:30:25 +0900

MDRでの公開時刻: 2024-09-03 08:30:25 +0900