# Phytochemical analysis and green synthesis of silver nanoparticles using Centella asiatica leaf and stem extracts: An investigation of antibacterial activity

https://mdr.nims.go.jp/datasets/daaeb234-b490-4a56-9c19-1da447ff1ef2

## Files

- [PLoS One_2025_20(9)_e0321172.pdf](https://mdr.nims.go.jp/filesets/6142e445-6a62-43ef-afa3-eff580fc6f9a/download) ([Detail](https://mdr.nims.go.jp/filesets/6142e445-6a62-43ef-afa3-eff580fc6f9a.md))

## Id

daaeb234-b490-4a56-9c19-1da447ff1ef2

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-10-09T01:46:49.584712Z

## Updated at

2025-10-21T07:05:38.733044Z

## Published at

2025-10-21T06:43:16.631785Z

## Doi



## First published url

https://doi.org/10.1371/journal.pone.0321172

## Date published

2025-09-25

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: 'Phytochemical analysis and green synthesis of silver nanoparticles using
    Centella asiatica leaf and stem extracts: An investigation of antibacterial activity'
  title_type: original
  lang: en

## Description

- description: "Centella asiatica (L.) Urban is a traditionally revered plant possessing
    several therapeutic\r\napplications. This research evaluated the phytochemical
    and antibacterial\r\nproperties of Centella asiatica. In addition, silver nanoparticles
    were synthesized\r\nusing the stem and leaf aqueous extracts. The presence of
    alkaloids, flavonoids,\r\nglycosides, terpenoids, and phenolics in plant extracts
    indicates their high medicinal\r\nvalue. Methanolic leaf extract showed a higher
    phenolic and flavonoid content\r\nof 43.73 ± 0.33 mg GAE/g and 19.76 ± 1.12 mg
    QE/g, respectively. It also contained\r\nthe most antioxidant activity with the
    lowest DPPH inhibitory concentration (IC50)\r\nof 49.31 ± 1.48 μg/mL. Plant extracts
    and synthesized nanoparticles were active\r\nagainst gram-positive and gram-negative
    bacteria. Methanolic leaf extract displayed\r\nan MIC of 27.5 and MBC of 55 mg/mL
    against S. aureus. Synthesized nanoparticles\r\nwere characterized using different
    spectroscopic techniques. UV-visible spectra\r\nof nanoparticles contained distinct
    absorption peaks resulting from surface plasmon\r\nresonance at 405 and 408 nm.
    The nanoparticles showed face-centered cubic\r\ncrystallinity in powder X-ray
    Diffraction analysis. Fourier Transfer Infrared spectra\r\nsuggested the possible
    involvement of organic functional groups in nanoparticle\r\nsynthesis. Field Emission
    Scanning Electron Microscopy and Transmission Electron\r\nMicroscopy analysis
    revealed the spherical shape with non-uniform size distribution.\r\nMean particle
    sizes were 20 nm and 19 nm for leaf and stem extract synthesized\r\nnanoparticles,
    respectively. In conclusion, Centella asiatica is rich in important plant\r\nsecondary
    metabolites and biological activities, and its aqueous extract synthesizes\r\nsilver
    nanoparticles that show potential antibacterial activity."
  description_type: abstract
  lang: eng

## Creator

- name: Kamana Sharma
  role: author
  organization: Tribhuvan University
- name: Manisha Bhusal
  role: author
  organization: Tribhuvan University
- name: Akash Budha Magar
  role: author
  organization: Tribhuvan University
- name: Ishwor Pathak
  role: author
  organization: Tribhuvan University
- name: SHRESTHA Lok Kumar
  role: author
  orcid: https://orcid.org/0000-0003-2680-6291
  organization: National Institute for Materials Science
  department: Research Center for Materials Nanoarchitectonics (MANA)/Nanomaterials
    Field/Supermolecules Group
- name: Khaga Raj Sharma
  role: author
  organization: Tribhuvan University

## Contact agent



## Publisher

organization: Public Library of Science

## Managing organization



## Keyword

- subject: Phytochemical analysis
  schema: not_defined
- subject: Centella asiatica (L.)
  schema: not_defined
- subject: Green synthesis
  schema: not_defined
- subject: Ag nanoparticles
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: PLoS One
  issn: '19326203'
  volume: '20'
  issue: '9'

## Conference



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## Instrument



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## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



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## Fileset

- id: 6142e445-6a62-43ef-afa3-eff580fc6f9a
  filename: PLoS One_2025_20(9)_e0321172.pdf
  content_type: application/pdf
  size: 2460660
  md5: 89619dbc75ec45974eb4a60ce8dd0c7d

## Thumbnail

fileset_id: 6142e445-6a62-43ef-afa3-eff580fc6f9a
filename: PLoS One_2025_20(9)_e0321172.pdf