# Mechanistic study of a low-power bacterial maintenance state using high-throughput electrochemistry

https://mdr.nims.go.jp/datasets/d3b78f65-a02f-41e5-bd18-94240df988b7

## File

- [GRANDFINAL-Manuscript.pdf](https://mdr.nims.go.jp/filesets/8a08404e-d3f4-4085-8f51-934f60cf542e/download) ([Detail](https://mdr.nims.go.jp/filesets/8a08404e-d3f4-4085-8f51-934f60cf542e.md))

## Id

d3b78f65-a02f-41e5-bd18-94240df988b7

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-12-16T04:43:45.964031Z

## Updated at

2025-10-22T23:30:36.268529Z

## Published at

2025-10-22T23:18:23.524143Z

## Doi

https://doi.org/10.48505/nims.5166

## First published url

https://doi.org/10.1016/j.cell.2024.09.042

## Date published

2024-10-23

## Recorded date published

2024-11

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Mechanistic study of a low-power bacterial maintenance state using high-throughput
    electrochemistry
  title_type: original
  lang: en

## Description

- description: "Mechanistic studies of life’s lower metabolic limits have been limited
    due to a paucity of tractable experimental systems. Here, we show that redox-cycling
    of phenazine-1-carboxamide (PCN) by Pseudomonas aeruginosa supports cellular maintenance
    in the absence of growth with a low mass-specific metabolic rate of 8.7 × 10−4
    W (g C)−1 at 25°C. Leveraging a high-throughput electrochemical culturing device,
    we find that non-growing cells cycling PCN tolerate conventional antibiotics but
    are susceptible to those that target membrane components. Under these conditions,
    cells conserve energy via a noncanonical, facilitated fermentation that is dependent
    on acetate kinase and NADH dehydrogenases. Across PCN concentrations that limit
    cell survival, the cell-specific metabolic rate is constant, indicating the cells
    are operating near their bioenergetic limit. This quantitative platform opens
    the door to further mechanistic investigations of maintenance, a physiological
    state that underpins microbial survival in nature and disease.\r\n"
  description_type: abstract
  lang: und

## Creator

- name: John A. Ciemniecki
  role: author
- name: Chia-Lun Ho
  role: author
- name: Richard D. Horak
  role: author
- name: Akihiro Okamoto
  role: author
  orcid: https://orcid.org/0000-0002-8102-4316
- name: Dianne K. Newman
  role: author
  orcid: https://orcid.org/0000-0003-1647-1918

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Phenazine
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-10-23
end_date: 2025-10-23

## Journal

- title: Cell
  issn: '00928674'
  volume: '187'
  issue: '24'
  start_page: 6882
  end_page: 6895

## Conference



## Related item



## Funding

- funder_name: National Institute of Allergy and Infectious Diseases
- funder_name: Japan Science and Technology Agency
- funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: 8a08404e-d3f4-4085-8f51-934f60cf542e
  filename: GRANDFINAL-Manuscript.pdf
  content_type: application/pdf
  size: 4254033
  md5: 2f86e2fa52937a303ebb717ba7fde331

## Thumbnail

fileset_id: 8a08404e-d3f4-4085-8f51-934f60cf542e
filename: GRANDFINAL-Manuscript.pdf