# Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner

https://mdr.nims.go.jp/datasets/ce519bdf-4414-4efe-ac41-82feaac7e0e2

## File

- [elife-101175-v1.pdf](https://mdr.nims.go.jp/filesets/9a14bb5f-5e47-4f2c-b68f-ab51b87e6180/download) ([Detail](https://mdr.nims.go.jp/filesets/9a14bb5f-5e47-4f2c-b68f-ab51b87e6180.md))
- [Supplementary File 01.pdf](https://mdr.nims.go.jp/filesets/e2b76eaf-a78e-4397-acc6-fdc6131b4f70/download) ([Detail](https://mdr.nims.go.jp/filesets/e2b76eaf-a78e-4397-acc6-fdc6131b4f70.md))
- [Supplementary File 02.pdf](https://mdr.nims.go.jp/filesets/1d97c2d8-6572-4a35-8c09-0ad675ce6f72/download) ([Detail](https://mdr.nims.go.jp/filesets/1d97c2d8-6572-4a35-8c09-0ad675ce6f72.md))
- [Supplementary File 03.pdf](https://mdr.nims.go.jp/filesets/eca983b7-1f88-4bda-a53f-0ca70bc09c29/download) ([Detail](https://mdr.nims.go.jp/filesets/eca983b7-1f88-4bda-a53f-0ca70bc09c29.md))

## Id

ce519bdf-4414-4efe-ac41-82feaac7e0e2

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-10-29T02:39:51.238056Z

## Updated at

2025-10-31T03:30:15.117513Z

## Published at

2025-10-31T03:24:38.551863Z

## Doi



## First published url

https://doi.org/10.7554/elife.101175

## Date published

2025-10-28

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Cell membrane glycan contents are biochemical factors that constitute a kinetic
    barrier to viral particle uptake in a protein-nonspecific manner
  title_type: original
  lang: en

## Description

- description: Various types of glycoproteins have been suggested to inhibit viral
    infection of cells via steric repulsion. However, it is difficult to evaluate
    such physical actions genetically, simply because they are nonspecific and can
    be caused by any molecule. Therefore, we investigated a method to compare this
    nonspecific action among cells with diverse membrane protein profiles. We found
    that a wide range of glycoproteins individually had a strong inhibitory effect
    on infection, while on the other hand, the total amount of glycans was negatively
    correlated with the infection level in each cell. Thus, the infection-inhibitory
    effect of glycoproteins was molecularly nonspecific, but was additively enhanced
    according to the amount of glycans on the cell surface. In this correlation, glycans
    function as a fundamental factor. Further investigating the mechanism by which
    glycans function as a factor in infection control, we conclude that the repulsion
    between proteins created by branched glycans forms a kinetic energy barrier against
    packing the virus into the region of protein interstitial space. As a result,
    the formation of the adhesive interface between the virus and the cell membrane,
    which is necessary for infection, is inhibited. This study attempted to link the
    cell's nonspecific physical properties with intracellular biochemicals. A similar
    approach may be applied to quantify other nonspecific biological phenomena.
  description_type: abstract
  lang: und

## Creator

- name: Yoshihisa Kaizuka
  role: author
  orcid: https://orcid.org/0000-0002-8019-0873
- name: Rika Machida
  role: author

## Contact agent



## Publisher

organization: eLife Sciences Publications, Ltd

## Managing organization



## Keyword

- subject: SARS-CoV-2
  schema: not_defined
- subject: whole genome glycocalyx prediction
  schema: not_defined
- subject: polymer brush theory
  schema: not_defined
- subject: superresolution
  schema: not_defined
- subject: cell virus interface
  schema: not_defined
- subject: FLIM-FRET
  schema: not_defined
- subject: protein Flory radius
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: eLife
  issn: 2050084X
  volume: '14'
  article_number: RP101175

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 9a14bb5f-5e47-4f2c-b68f-ab51b87e6180
  filename: elife-101175-v1.pdf
  content_type: application/pdf
  size: 11438312
  md5: 9be6867f1ce1683e5b7f04eec9175bbc
- id: e2b76eaf-a78e-4397-acc6-fdc6131b4f70
  filename: Supplementary File 01.pdf
  content_type: application/pdf
  size: 103316
  md5: 91bb5e827cf521d5838dfbc5cd77c20b
- id: 1d97c2d8-6572-4a35-8c09-0ad675ce6f72
  filename: Supplementary File 02.pdf
  content_type: application/pdf
  size: 24185171
  md5: 2c95947de2fb9fc2c25ad8cc72cfedfc
- id: eca983b7-1f88-4bda-a53f-0ca70bc09c29
  filename: Supplementary File 03.pdf
  content_type: application/pdf
  size: 30238
  md5: 88eb0c689d539bb6936807c162412c23

## Thumbnail

fileset_id: 9a14bb5f-5e47-4f2c-b68f-ab51b87e6180
filename: elife-101175-v1.pdf