# Reversible Solubility Switching of a Polymer Triggered by Visible‐Light Responsive Azobenzene Photochromism with Negligible Thermal Relaxation

https://mdr.nims.go.jp/datasets/ccee48be-aad2-48e1-a7a6-b7b8a04546e6

## File

- [240726_R1_Osaka_manuscript.docx](https://mdr.nims.go.jp/filesets/275e0ac1-5a09-43f8-9b14-7e20dc7d657b/download) ([Detail](https://mdr.nims.go.jp/filesets/275e0ac1-5a09-43f8-9b14-7e20dc7d657b.md))

## Id

ccee48be-aad2-48e1-a7a6-b7b8a04546e6

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-09-03T04:14:24.045204Z

## Updated at

2024-12-04T05:52:28.711236Z

## Published at

2024-12-04T05:52:29.983516Z

## Doi

https://doi.org/10.48505/nims.5075

## First published url

https://doi.org/10.1002/marc.202400419

## Date published

2024-08-08

## Recorded date published

2024-11

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Reversible Solubility Switching of a Polymer Triggered by Visible‐Light Responsive
    Azobenzene Photochromism with Negligible Thermal Relaxation
  title_type: original
  lang: en

## Description

- description: This study reports the reversible solubility switching of a polymer
    triggered by non-phototoxic visible light. A photochromic polymerizable azobenzene
    monomer with four methoxy groups at the ortho-position (mAzoA) was synthesized,
    exhibiting reversible photoisomerization between transand cis-states using green
    (546 nm) and blue light (436 nm). Free radical copolymerization of hydrophilic
    dimethylacrylamide (DMAAm) with mAzoA produced a light-responsive random copolymer
    (P(mAzoA-r-DMAAm)) that shows a reversible photochromic reaction to visible light.
    Optimizing mAzoA content resulted in P(mAzoA10.7-r-DMAAm)3.0 kDa exhibiting LCST-type
    phase separation in PBS (pH 7.4) with transand cis-states at 39.2 °C and 32.9
    °C, respectively. The bistable temperature range of 6.3 °C covers 37 °C, suitable
    for mammalian cell culture. Reversible solubility changes were demonstrated under
    alternating green and blue light at 37 °C. 1H NMR indicated significant retardation
    of thermal relaxation from cisto trans-states, preventing undesired thermal mechanical
    degradation. Madin Darby Canine Kidney (MDCK) cells adhered to the P(mAzoA-r-DMAAm)
    hydrogel, confirming its non-cytotoxicity and potential for biocompatible interfaces.
    This principle is useful for developing hydrogels that can reversibly stimulate
    cells mechanically or chemically in response to visible light.
  description_type: abstract
  lang: und

## Creator

- name: Takeshi Ueki
  role: author
  orcid: https://orcid.org/0000-0001-9317-6280
- name: Yuna Osaka
  role: author
- name: Kenta Homma
  role: author
  orcid: https://orcid.org/0000-0002-3960-4093
- name: Shota Yamamoto
  role: author
  orcid: https://orcid.org/0000-0002-7422-0968
- name: Aya Saruwatari
  role: author
  orcid: https://orcid.org/0000-0001-7388-4626
- name: Hongxin Wang
  role: author
  orcid: https://orcid.org/0000-0002-8984-0764
- name: Masao Kamimura
  role: author
  orcid: https://orcid.org/0000-0001-8510-2935
- name: Jun Nakanishi
  role: author
  orcid: https://orcid.org/0000-0003-4457-6581

## Contact agent



## Publisher

organization: Wiley

## Managing organization



## Keyword

- subject: azobenzene, gels. LCST, mechanobiology, phase transition
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Macromolecular Rapid Communications
  issn: '10221336'
  volume: '45'
  issue: '22'
  article_number: '2400419'

## Conference



## Related item



## Funding

- identifier: 20H02804
  funder_name: Japan Society for the Promotion of Science
- identifier: 20K21229
  funder_name: Japan Society for the Promotion of Science
- identifier: 23H02030
  funder_name: Japan Society for the Promotion of Science
- identifier: 22K14705
  funder_name: Japan Society for the Promotion of Science
- identifier: 22H00596
  funder_name: Japan Society for the Promotion of Science
- identifier: 23K17481
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: 275e0ac1-5a09-43f8-9b14-7e20dc7d657b
  filename: 240726_R1_Osaka_manuscript.docx
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  size: 3562862
  md5: e2f2f411f3e3db25491319584d9f9f5a

## Thumbnail

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filename: 240726_R1_Osaka_manuscript.docx