# Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting probe

https://mdr.nims.go.jp/datasets/cb92fab6-17e9-4ce3-8ff2-35548b956948

## File

- [sciadv.adz6633.pdf](https://mdr.nims.go.jp/filesets/31f4f886-fe41-48b7-ad74-3c62f5a2c423/download) ([Detail](https://mdr.nims.go.jp/filesets/31f4f886-fe41-48b7-ad74-3c62f5a2c423.md))

## Id

cb92fab6-17e9-4ce3-8ff2-35548b956948

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-10-27T05:10:57.420963Z

## Updated at

2025-10-28T03:30:18.740106Z

## Published at

2025-10-28T03:16:23.866995Z

## Doi



## First published url

https://doi.org/10.1126/sciadv.adz6633

## Date published

2025-10-24

## Recorded date published

2025-10-24

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA–targeting
    probe
  title_type: original
  lang: en

## Description

- description: "Early detection and late-stage cell fate assessment are key factors
    to develop therapeutic\r\nstrategies, although current methods cannot capture
    early responses or distinguish multiple injury\r\nstates, especially in UV-vis-sensitive
    cells. Here, we introduce a method to simultaneously detect\r\nvariations in RNA
    and DNA under near-infrared photoexcitation. Utilizing a pyrazinacene-based \r\nprobe
    (TEG8-N14), we unexpectedly achieved discrimination of multiple cell states, including
    \r\napoptosis, necrosis, necroptosis, and senescence, based on RNA/DNA changes.
    Specifically, TEG8- \r\nN14 selectively stains necrotic cells in live samples,
    while after fixation, it allows detection of ultra-\r\nearly senescence in UV-vis-sensitive
    cells, providing approximately two-fold greater informational \r\ncontent than
    existing RNA or DNA fluorophores. These findings break current imaging barriers
    by\r\nenabling comprehensive visualization of single-cell fate histories without
    being affected by UV-vis \r\nor genetic manipulation."
  description_type: abstract
  lang: und

## Creator

- name: Linawati Sutrisno
  role: author
  orcid: https://orcid.org/0000-0003-3085-9660
- name: Gary J. Richards
  role: author
  orcid: https://orcid.org/0000-0002-5262-2587
- name: Jack D. Evans
  role: author
  orcid: https://orcid.org/0000-0001-9521-2601
- name: Michio Matsumoto
  role: author
  orcid: https://orcid.org/0000-0002-6037-0228
- name: Xianglan Li
  role: author
  orcid: https://orcid.org/0000-0003-0992-1722
- name: Koichiro Uto
  role: author
  orcid: https://orcid.org/0000-0001-7091-0585
- name: Jonathan P. Hill
  role: author
  orcid: https://orcid.org/0000-0002-4229-5842
- name: Masayasu Taki
  role: author
  orcid: https://orcid.org/0000-0003-2185-4593
- name: Shigehiro Yamaguchi
  role: author
  orcid: https://orcid.org/0000-0003-0072-8969
- name: Katsuhiko Ariga
  role: author
  orcid: https://orcid.org/0000-0002-2445-2955

## Contact agent



## Publisher

organization: American Association for the Advancement of Science (AAAS)

## Managing organization



## Keyword

- subject: RNA/DNA
  schema: not_defined
- subject: N-heteroacene
  schema: not_defined
- subject: senescence
  schema: not_defined
- subject: near-infrared
  schema: not_defined
- subject: UV-vis sensitive
  schema: not_defined
- subject: Mutiplex labeling
  schema: not_defined
- subject: Bioimaging
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Science Advances
  issn: '23752548'
  volume: '11'
  issue: '43'
  article_number: eadz6633

## Conference



## Related item



## Funding

- identifier: JP24KF0089
  funder_name: JSPS
- identifier: JP25K23571
  funder_name: JSPS
- identifier: JP21K05044
  funder_name: JSPS
- identifier: DE220100163
  funder_name: Australian Research Council Discovery Early Career Award

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## Fileset

- id: 31f4f886-fe41-48b7-ad74-3c62f5a2c423
  filename: sciadv.adz6633.pdf
  content_type: application/pdf
  size: 4058494
  md5: 461d5f5e59fdf985ccc179b293589d3a

## Thumbnail

fileset_id: 31f4f886-fe41-48b7-ad74-3c62f5a2c423
filename: sciadv.adz6633.pdf