# Electrochemical Characterization of Two Gut Microbial Strains Cooperatively Promoting Multiple Sclerosis Pathogenesis

https://mdr.nims.go.jp/datasets/c8bbf4f0-d8ae-429f-b22f-600ce51492a2

## File

- [Divya et al 2024 Two gut microbial strains Multiple Sclerosis Pathogenesis microorganisms-12-00257.pdf](https://mdr.nims.go.jp/filesets/ba7642ef-a0d2-4a44-8cb1-97868a66c9e4/download) ([Detail](https://mdr.nims.go.jp/filesets/ba7642ef-a0d2-4a44-8cb1-97868a66c9e4.md))

## Id

c8bbf4f0-d8ae-429f-b22f-600ce51492a2

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-11-04T18:59:10.371271Z

## Updated at

2024-12-09T07:30:22.216109Z

## Published at

2024-12-09T07:30:22.541685Z

## Doi



## First published url

https://doi.org/10.3390/microorganisms12020257

## Date published

2024-01-25

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Electrochemical Characterization of Two Gut Microbial Strains Cooperatively
    Promoting Multiple Sclerosis Pathogenesis
  title_type: original
  lang: en

## Description

- description: In this study, we explored the extracellular electron transfer (EET)
    capabilities of two bacterial strains, OTU0001 and OTU0002, which are demonstrated
    in biofilm formation in mouse gut and the induction of autoimmune diseases like
    multiple sclerosis. OTU0002 displayed significant electrogenic behaviour, producing
    microbial current on an indium tin-doped oxide electrode surface, particularly
    in the presence of glucose, with a current density of 60 nA/cm2. The presence
    of cell-surface redox substrate potentially mediating EET was revealed by the
    redox-based staining method and electrochemical voltammetry assay. However, medium
    swapping analyses and the addition of flavins, a model redox mediator, suggest
    that the current production is dominated by soluble endogenous redox substrates
    in OTU0002. Given redox substrates were detected at the cell surface, the secreted
    redox molecule may interact with the cellular surface of OTU0002. In contrast
    to OTU0002, OTU0001 did not exhibit notable electrochemical activity, lacking
    cell-surface redox molecules. Further, the mixture of the two strains did not
    increase the current production from OTU0001, suggesting that OTU0001 does not
    support the EET mechanism of OTU0002. The present work revealed the coexistence
    of EET and non-EET capable pathogens in multi-species biofilm.
  description_type: abstract
  lang: und

## Creator

- name: Divya Naradasu
  role: author
- name: Waheed Miran
  role: author
- name: Akihiro Okamoto
  role: author
  orcid: https://orcid.org/0000-0002-8102-4316
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: Whole-cell electrochemistry
  schema: not_defined
- subject: Gut microbes
  schema: not_defined
- subject: Extracellular electron transfer
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Microorganisms
  issn: '20762607'
  volume: '12'
  issue: '2'
  article_number: '257'

## Conference



## Related item



## Funding

- identifier: 20H05590
  funder_name: Japan Society for Promotion of Science KAKENHI
- identifier: 22H02265
  funder_name: Japan Society for Promotion of Science KAKENHI
- identifier: 19gm6010002h0004
  funder_name: Japan Agency for Medical Research and Development
- identifier: 22ae0121044h0002
  funder_name: Japan Agency for Medical Research and Development
- identifier: JPMJPR19H1
  funder_name: JST
- identifier: JPMJCR19H4
  funder_name: CREST

## Instrument



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## Measurement method



## Specimen



## Chemical composition



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## Fileset

- id: ba7642ef-a0d2-4a44-8cb1-97868a66c9e4
  filename: Divya et al 2024 Two gut microbial strains Multiple Sclerosis Pathogenesis
    microorganisms-12-00257.pdf
  content_type: application/pdf
  size: 2898139
  md5: 63703096828b5ad5bb1b35d9fd09231f

## Thumbnail

fileset_id: ba7642ef-a0d2-4a44-8cb1-97868a66c9e4
filename: Divya et al 2024 Two gut microbial strains Multiple Sclerosis Pathogenesis
  microorganisms-12-00257.pdf