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In this study, we explored the extracellular electron transfer (EET) capabilities of two bacterial strains, OTU0001 and OTU0002, which are demonstrated in biofilm formation in mouse gut and the induction of autoimmune diseases like multiple sclerosis. OTU0002 displayed significant electrogenic behaviour, producing microbial current on an indium tin-doped oxide electrode surface, particularly in the presence of glucose, with a current density of 60 nA/cm2. The presence of cell-surface redox substrate potentially mediating EET was revealed by the redox-based staining method and electrochemical voltammetry assay. However, medium swapping analyses and the addition of flavins, a model redox mediator, suggest that the current production is dominated by soluble endogenous redox substrates in OTU0002. Given redox substrates were detected at the cell surface, the secreted redox molecule may interact with the cellular surface of OTU0002. In contrast to OTU0002, OTU0001 did not exhibit notable electrochemical activity, lacking cell-surface redox molecules. Further, the mixture of the two strains did not increase the current production from OTU0001, suggesting that OTU0001 does not support the EET mechanism of OTU0002. The present work revealed the coexistence of EET and non-EET capable pathogens in multi-species biofilm.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Whole-cell electrochemistry, Gut microbes, Extracellular electron transfer

刊行年月日: 2024-01-25

出版者: MDPI AG

掲載誌:

• Microorganisms (ISSN: 20762607) vol. 12 issue. 2 257

研究助成金:

• Japan Society for Promotion of Science KAKENHI 20H05590
• Japan Society for Promotion of Science KAKENHI 22H02265
• Japan Agency for Medical Research and Development 19gm6010002h0004
• Japan Agency for Medical Research and Development 22ae0121044h0002
• JST JPMJPR19H1
• CREST JPMJCR19H4

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.3390/microorganisms12020257

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更新時刻: 2024-12-09 16:30:22 +0900

MDRでの公開時刻: 2024-12-09 16:30:22 +0900