# Potential-Switchable Viscoelasticity of Protein Nanolayers at a Liquid/Liquid Interface

https://mdr.nims.go.jp/datasets/be3eb78a-d5b8-4887-86dd-393bca897f7c

## File

- [[Langmuir]Potential-switchable viscoelasticity of protein nanolayers at a liquid liquid interface.pdf](https://mdr.nims.go.jp/filesets/15ad5176-6629-46d7-af46-607b4b904861/download) ([Detail](https://mdr.nims.go.jp/filesets/15ad5176-6629-46d7-af46-607b4b904861.md))

## Id

be3eb78a-d5b8-4887-86dd-393bca897f7c

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-09-02T04:16:03.094459Z

## Updated at

2025-09-02T07:30:27.983662Z

## Published at

2025-09-02T07:19:01.611635Z

## Doi



## First published url

https://doi.org/10.1021/acs.langmuir.5c01819

## Date published

2025-07-15

## Recorded date published

2025-7-15

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Potential-Switchable Viscoelasticity of Protein Nanolayers at a Liquid/Liquid
    Interface
  title_type: original
  lang: en

## Description

- description: Protein nanolayers(PNLs) formed at an electrochemical liquid|liquid
    interface between water (W) and a fluorous solvent (F) were examined by using
    interfacial rheological measurement (IRM) and neutron reflectometry (NR) under
    the externally controlled condition of the phase boundary potential differences
    EF W(=φW−φF + const.), where F contained a hydrophobic ionic liquid (IL) as a
    supporting electrolyte and W, whose pH was 7.4, contained a protein, bovine serum
    albumin (BSA).The IRM and NR results illuminated  that both static and dynamic
    properties of the PNL at the electrochemical F|W interface were varied by applying
    EF W. NR found minimal EF W dependence on the adsorption amount of BSA in the
    PNL. In contrast, IRM revealed that although the interfacial shear loss moduli
    G″ of the PNL was constant regardless of EF W, the interfacial shear storage G′
    of the PNL increased dramatically at more negative EF W, showing a more elastic
    response.This difference between static and dynamic properties results from the
    increase in intermolecular and intramolecular interactions between BSA molecules
    in the PNL at more negative EF W due to the accelerated denaturation of negatively
    charged BSA that formed complexes with IL cations accumulated on the F side of  the
    F|W interface. The G′ and G″ reversibly responded to switching between different
    potentials (a positive and a negative EF W).These IRM results unveiled that the
    viscoelasticity of  the PNL at the electrochemical F|W interface is reversibly
    potential-switchable.The present interface-specific method using the potential
    control is a new promising method to  diversify and switch the PNL structure reversibly.
    The reversible structural control of the PNL would enable us to perform real-time
    observation of cells reacting to environmental changes at liquid|liquid interfaces.
  description_type: abstract
  lang: und

## Creator

- name: Kosuke Ishii
  role: author
- name: Takeshi Ueki
  role: author
  orcid: https://orcid.org/0000-0001-9317-6280
- name: Jun Nakanishi
  role: author
  orcid: https://orcid.org/0000-0003-4457-6581
- name: Kazuhiro Akutsu-Suyama
  role: author
  orcid: https://orcid.org/0000-0002-4797-6604
- name: Norifumi L. Yamada
  role: author
  orcid: https://orcid.org/0000-0002-8370-4526
- name: Yuko Yokoyama
  role: author
  orcid: https://orcid.org/0000-0002-3943-0978
- name: Tetsuo Sakka
  role: author
  orcid: https://orcid.org/0000-0002-1892-8056
- name: Naoya Nishi
  role: author
  orcid: https://orcid.org/0000-0002-5654-5603

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: ionic liquid
  schema: not_defined
- subject: interface
  schema: not_defined
- subject: electrochemistry
  schema: not_defined
- subject: viscoelasticity
  schema: not_defined
- subject: cell
  schema: not_defined
- subject: mechanobiology
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Langmuir
  issn: '07437463'
  volume: '41'
  issue: '27'
  start_page: 17973
  end_page: 17981

## Conference



## Related item



## Funding

- identifier: 23H02030
  funder_name: Japan Society for the Promotion of Science
- identifier: 23H03829
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 15ad5176-6629-46d7-af46-607b4b904861
  filename: "[Langmuir]Potential-switchable viscoelasticity of protein nanolayers
    at a liquid liquid interface.pdf"
  content_type: application/pdf
  size: 2820027
  md5: 25a5736efd1b4803187392b558bb7810

## Thumbnail

fileset_id: 15ad5176-6629-46d7-af46-607b4b904861
filename: "[Langmuir]Potential-switchable viscoelasticity of protein nanolayers at
  a liquid liquid interface.pdf"