# Effective disruption of cancer cell membranes by photodynamic therapy with cell membrane-adhesive photosensitizer

https://mdr.nims.go.jp/datasets/bcdd2a71-82f7-4d00-9f9b-1a87d8c225c3

## File

- [Effective disruption of cancer cell membranes by photodynamic therapy with cell membrane-adhesive photosensitizer.pdf](https://mdr.nims.go.jp/filesets/b4664e6c-869f-4f0a-9a51-4ca00c2193a9/download) ([Detail](https://mdr.nims.go.jp/filesets/b4664e6c-869f-4f0a-9a51-4ca00c2193a9.md))

## Id

bcdd2a71-82f7-4d00-9f9b-1a87d8c225c3

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-11-24T05:22:51.876596Z

## Updated at

2024-11-25T07:30:47.476933Z

## Published at

2024-11-25T07:30:47.544950Z

## Doi



## First published url

https://doi.org/10.3164/jcbn.24-88

## Date published

2024-08-23

## Recorded date published

2024

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Effective disruption of cancer cell membranes by photodynamic therapy with
    cell membrane-adhesive photosensitizer
  title_type: original
  lang: en

## Description

- description: Photodynamic therapy (PDT) is a non-invasive cancer treatment modality
    that entails the administration of photosensitizers and light irradiation. Previously,
    we have established a polycation-containing hematoporphyrin (aHP) formulation,
    demonstrating superior anti-tumor efficacy compared to original hematoporphyrin
    (HP) in vivo. To reveal the variance in anti-tumor efficacy between them, we conducted
    timelapse imaging of rat gastric mucosa cell-line (RGM1) and its cancerous derivative
    (RGK45) treated with the porphyrins, and also analyzed their cellular localization
    and glycan chain expression on cellular membranes. Timelapse imaging of RGK45
    treated with aHP revealed swelling, rupturing of cells, and subsequent scattering
    of small vesicles upon light irradiation, contrasting with the unchanged morphology
    of RGK45 treated with HP throughout the same irradiation duration. Furthermore,
    aHP exhibited a more concentrated cellular localization on cell membranes compared
    to HP with more diffuse fluorescence within cells. Additionally, neither aHP nor
    HP induced swelling or rupturing of cellular membranes in RGM1. Flow cytometry
    analysis demonstrated significantly higher fluorescence of wheat germ agglutinin
    conjugated dye in RGK45 compared to RGM1 (* p < 0.05), suggesting differential
    glycan expression patterns. These findings collectively suggest that the cellular
    toxicity of aHP may be augmented in RGK45 due to its heightened affinity towards
    negatively charged structures on cellular membranes and its preferential localization
    on them. The observed membrane rupturing and release of vesicles may confer an
    abscopal effect in addition to its direct PDT effect, thereby positioning aHP
    as a promising next-generation photosensitizer.
  description_type: abstract
  lang: und

## Creator

- name: Aoi Hoshi
  role: author
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Yoshiki Komatsu
  role: author
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Hiroko Bando
  role: author
- name: Hisato Hara
  role: author
- name: Hirofumi Matsui
  role: author

## Contact agent



## Publisher

organization: The Society for Free Radical Research Japan

## Managing organization



## Keyword

- subject: photodynamic therapy
  schema: not_defined
- subject: hematoporphyrin
  schema: not_defined
- subject: polycation
  schema: not_defined
- subject: cell adhesive
  schema: not_defined
- subject: cellular membrane
  schema: not_defined
- subject: glycan
  schema: not_defined

## Rights

- description: "This is an open access article distributed under the terms of the
    Creative Commons Attribution-NonCommercial-NoDerivatives License.\r\nhttps://creativecommons.org/licenses/by-nc-nd/4.0/"
  identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Journal of Clinical Biochemistry and Nutrition
  issn: '09120009'
  volume: '75'
  issue: '3'
  start_page: 197
  end_page: 203
  article_number: 24-88

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## Specimen



## Chemical composition



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## Fileset

- id: b4664e6c-869f-4f0a-9a51-4ca00c2193a9
  filename: Effective disruption of cancer cell membranes by photodynamic therapy
    with cell membrane-adhesive photosensitizer.pdf
  content_type: application/pdf
  size: 1264662
  md5: c6f661142b3a0919a85c0a2ddf931704

## Thumbnail

fileset_id: b4664e6c-869f-4f0a-9a51-4ca00c2193a9
filename: Effective disruption of cancer cell membranes by photodynamic therapy with
  cell membrane-adhesive photosensitizer.pdf