# Distinct macrophage uptake of engineered and biological particles driven by host age and sex

https://mdr.nims.go.jp/datasets/bbabbd10-f452-4980-ae0f-615e38ca6acd

## Files

- [Distinct macrophage uptake of engineered and biological particles driven by host age and sex.pdf](https://mdr.nims.go.jp/filesets/8be383ba-2d7d-4629-8a07-d01194ebe1e7/download) ([Detail](https://mdr.nims.go.jp/filesets/8be383ba-2d7d-4629-8a07-d01194ebe1e7.md))
- [Revised_Supp_for_STAM.pdf](https://mdr.nims.go.jp/filesets/6bb2a9f4-7792-40d1-a5d2-f049cbc9d9b2/download) ([Detail](https://mdr.nims.go.jp/filesets/6bb2a9f4-7792-40d1-a5d2-f049cbc9d9b2.md))
- [STAM-2025-0435_data.zip](https://mdr.nims.go.jp/filesets/b43e93f4-00ad-4e9a-929b-0af7380ee52d/download) ([Detail](https://mdr.nims.go.jp/filesets/b43e93f4-00ad-4e9a-929b-0af7380ee52d.md))

## Id

bbabbd10-f452-4980-ae0f-615e38ca6acd

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-01-16T04:35:59.954784Z

## Updated at

2026-02-03T06:03:39.382299Z

## Published at

2026-01-19T03:21:48.143431Z

## Doi

https://doi.org/10.48505/nims.6138

## First published url

https://doi.org/10.1080/14686996.2025.2610875

## Date published

2026-12-31

## Recorded date published

2026-12-31

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Distinct macrophage uptake of engineered and biological particles driven
    by host age and sex
  title_type: original
  lang: en

## Description

- description: Understanding how nano- and microparticles interact with biological
    systems is essential for advancing the biomedical applications of engineered materials.
    These interactions are governed not only by the physicochemical properties of
    the particles – such as size, shape, and surface chemistry – but also by host-specific
    physiological factors. However, how intrinsic host factors, particularly age and
    biological sex, affect interactions between immune cells and particles remains
    poorly understood. In this study, we systematically investigated how these intrinsic
    host variables influence the cellular uptake of polymeric particles by primary
    macrophages. Using a library of particles with controlled sizes (25, 250, and
    3000 nm) and surface chemistries (unmodified, amine-, and carboxyl-functionalized),
    as well as with biological particles (bacteria and yeast), we compared uptake
    efficiencies in macrophages derived from male and female mice of various ages.
    We observed significant age- and sex-dependent differences in particle internalization.
    Transcriptomic profiling revealed differentially expressed genes related to receptor-mediated
    endocytosis and actin cytoskeleton remodeling, suggesting that molecular mechanisms
    underly these variations. Additionally, protein coronas were formed by incubating
    polymeric particles with autologous serum, revealing sex-dependent differences
    in corona composition and resulting macrophage recognition. Our findings highlight
    the critical interplay between engineered-material properties and host biological
    variability. Accordingly, this work provides key insights for the rational design
    of nanomaterials tailored to perform consistently across heterogeneous biological
    populations, thereby advancing the development of personalized nanomedicine and
    immunomodulatory materials.
  description_type: abstract
  lang: en

## Creator

- name: Riki Toita
  role: author
  organization: National Institute of Advanced Industrial Science and Technology (AIST)
  department: a Molecular Biosystems Research Institute
- name: Yuki Shimizu
  role: author
- name: Jeong-Hun Kang
  role: author

## Contact agent



## Publisher

organization: Taylor & Francis

## Managing organization



## Keyword

- subject: Word
  schema: not_defined
- subject: nanomedicines
  schema: not_defined
- subject: aging
  schema: not_defined
- subject: biological sex
  schema: not_defined
- subject: macrophages
  schema: not_defined
- subject: uptake
  schema: not_defined
- subject: RNA-seq
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Science and Technology of Advanced Materials
  issn: '14686996'
  volume: '27'
  article_number: '2610875'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 8be383ba-2d7d-4629-8a07-d01194ebe1e7
  filename: Distinct macrophage uptake of engineered and biological particles driven
    by host age and sex.pdf
  content_type: application/pdf
  size: 5730775
  md5: 2611eb80b2c16d67b99dd47b448dff71
- id: 6bb2a9f4-7792-40d1-a5d2-f049cbc9d9b2
  filename: Revised_Supp_for_STAM.pdf
  content_type: application/pdf
  size: 1032262
  md5: 441dd17635c74520732ed55c01d9f5af
- id: b43e93f4-00ad-4e9a-929b-0af7380ee52d
  filename: STAM-2025-0435_data.zip
  content_type: application/zip
  size: 25582
  md5: 4d382e6a7acefce40893d2737772bf7d

## Thumbnail

fileset_id: 8be383ba-2d7d-4629-8a07-d01194ebe1e7
filename: Distinct macrophage uptake of engineered and biological particles driven
  by host age and sex.pdf