# Enhanced Cancer Immunotherapy via Synergistic Action of NO-Donor Nanoparticles (NanoARG) and PD-1 Antibody

https://mdr.nims.go.jp/datasets/bb7239fb-9d46-44cf-86d8-ba94fafd6695

## File

- [Enhanced cancer immunotherapy via synergistic action of NO-Donor nanoparticles  NanoARG  and PD-1 antibody.pdf](https://mdr.nims.go.jp/filesets/4aa8a4bf-deaa-4555-9f14-ced8c9470ed0/download) ([Detail](https://mdr.nims.go.jp/filesets/4aa8a4bf-deaa-4555-9f14-ced8c9470ed0.md))
- [STAM-2025-0045data.zip](https://mdr.nims.go.jp/filesets/79d8bea1-2436-4d8d-b2f0-d652a099e23a/download) ([Detail](https://mdr.nims.go.jp/filesets/79d8bea1-2436-4d8d-b2f0-d652a099e23a.md))

## Id

bb7239fb-9d46-44cf-86d8-ba94fafd6695

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-07-31T04:20:59.775066Z

## Updated at

2025-07-31T23:30:21.108136Z

## Published at

2025-07-31T23:16:47.430567Z

## Doi

https://doi.org/10.48505/nims.5621

## First published url

https://doi.org/10.1080/14686996.2025.2538430

## Date published

2025-12-31

## Recorded date published

2025-12-31

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Enhanced Cancer Immunotherapy via Synergistic Action of NO-Donor Nanoparticles
    (NanoARG) and PD-1 Antibody
  title_type: original
  lang: en

## Description

- description: 'This study explores the synergistic potential of PEG-b-P(L-Arg)-based
    polyion complex micelles (NanoARGs) combined with an immune checkpoint inhibitor
    (PD-1 antibody) for cancer immunotherapy. Comprehensive experiments, including
    micelle preparation, in vivo anti-tumor activity evaluation, nitric oxide (NO)
    quantification, and immunofluorescence analysis, revealed significant insights.
    NanoARGs exhibited a biphasic effect on tumor growth: high doses inhibited tumor
    growth through NO generated from liberated Arg, whereas low doses promoted tumor
    progression. The combination treatment demonstrated significant synergistic anti-tumor
    activity without notable adverse effects, and treated mice tolerated the regimen
    well. This approach elevated NO levels in serum and tumor tissues, enhanced immune
    cell infiltration into tumor tissues, and facilitated the polarization of tumor-associated
    macrophages to the M1 phenotype. PD-1 antibody further amplified these effects
    by blocking PD-1/PD-L1 interactions and reactivating T cells. These results underscore
    the therapeutic potential of this novel approach, providing a foundation for optimizing
    tumor immunotherapy strategies and advancing clinical applications. Future research
    will focus on elucidating the mechanisms of action and expanding the scope of
    this promising treatment.'
  description_type: abstract
  lang: en

## Creator

- name: Mei Ting
  role: author
  organization: Beijing University of Chinese Medicine
  department: a School of Chinese Materia Medica
- name: Xin Zhang
  role: author
- name: Xiaolu Hou
  role: author
- name: Yukio Nagasaki
  role: author

## Contact agent



## Publisher

organization: Taylor & Francis

## Managing organization



## Keyword

- subject: nanoparticle-based nitric oxide donor
  schema: not_defined
- subject: immune checkpoint inhibitor (PD-1 antibody)
  schema: not_defined
- subject: cancer immunotherapy
  schema: not_defined
- subject: synergistic anti-tumor effect
  schema: not_defined
- subject: Poly(L-arginine)-based polymer micelles
  schema: not_defined
- subject: Tumor-associated macrophages
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Science and Technology of Advanced Materials
  issn: '14686996'
  volume: '26'
  article_number: '2538430'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 4aa8a4bf-deaa-4555-9f14-ced8c9470ed0
  filename: Enhanced cancer immunotherapy via synergistic action of NO-Donor nanoparticles  NanoARG  and
    PD-1 antibody.pdf
  content_type: application/pdf
  size: 5341749
  md5: 419dfaf10483cebf8dbfc32a28dbac35
- id: 79d8bea1-2436-4d8d-b2f0-d652a099e23a
  filename: STAM-2025-0045data.zip
  content_type: application/zip
  size: 5937788
  md5: ea95cd32a986117137ee49abf3b6d3c0

## Thumbnail

fileset_id: 4aa8a4bf-deaa-4555-9f14-ced8c9470ed0
filename: Enhanced cancer immunotherapy via synergistic action of NO-Donor nanoparticles  NanoARG  and
  PD-1 antibody.pdf