# Resolution improvement of differential phase-contrast microscopy via tilt-series acquisition for environmental cell application

https://mdr.nims.go.jp/datasets/ba4806b0-53da-4d95-b0b4-fb9e7831d047

## File

- [Resolution Improvement of DPC Microscopy_R1_ preprint.pdf](https://mdr.nims.go.jp/filesets/be65dbc9-f11b-46f7-9805-12bb6b215a61/download) ([Detail](https://mdr.nims.go.jp/filesets/be65dbc9-f11b-46f7-9805-12bb6b215a61.md))

## Id

ba4806b0-53da-4d95-b0b4-fb9e7831d047

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-11-07T06:59:23.014011Z

## Updated at

2025-11-10T23:30:20.373531Z

## Published at

2025-11-10T23:22:14.758165Z

## Doi

https://doi.org/10.48505/nims.5884

## First published url

https://doi.org/10.1093/jmicro/dfae049

## Date published

2025-03-31

## Recorded date published

2025-3-31

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Resolution improvement of differential phase-contrast microscopy via tilt-series
    acquisition for environmental cell application
  title_type: original
  lang: en

## Description

- description: A simple method that improves the resolution of the phase measurement
    of differential phase contrast (DPC) scanning transmission electron microscopy
    for closed-type environmental cell applications was developed and tested using
    a model sample simulating environmental cell observations. Because the top and
    bottom membranes of an environmental cell are typically far apart, the images
    from these embranes are shifted widely by tilt-series acquisition, and averaging
    the images after alignment can effectively  eliminate undesired signals from the
    membranes while improving the signal from the object of interest. It was demonstrated
    that a phase precision of 2π/100 rad is well achievable using the proposed method
    for the sample in an environmental cell.
  description_type: abstract
  lang: und

## Creator

- name: Kazutaka Mitsuishi
  role: author
  orcid: https://orcid.org/0000-0002-9361-4057
  organization: National Institute for Materials Science
- name: Fumiaki Ichihashi
  role: author
- name: Yoshio Takahashi
  role: author
- name: Katsuaki Nakazawa
  role: author
  orcid: https://orcid.org/0000-0002-6056-5615
  organization: National Institute for Materials Science
- name: Masaki Takeguchi
  role: author
  orcid: https://orcid.org/0000-0002-0282-6020
  organization: National Institute for Materials Science
- name: Ayako Hashimoto
  role: author
  orcid: https://orcid.org/0000-0002-1985-7667
  organization: National Institute for Materials Science
- name: Toshiaki Tanigaki
  role: author

## Contact agent



## Publisher

organization: Oxford University Press (OUP)

## Managing organization



## Keyword

- subject: differential phase contrast
  schema: not_defined
- subject: environmental cell
  schema: not_defined
- subject: in situ transmission electron microscopy
  schema: not_defined

## Rights

- identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-10-16
end_date: 2025-10-16

## Journal

- title: Microscopy
  issn: '20505701'
  volume: '74'
  issue: '2'
  start_page: 92
  end_page: 97

## Conference



## Related item



## Funding

- identifier: JPJ004596
  funder_name: Innovative Science and Technology Initiative for Security
- identifier: JPJ004596
  funder_name: Innovative Science and Technology Initiative for Security

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: be65dbc9-f11b-46f7-9805-12bb6b215a61
  filename: Resolution Improvement of DPC Microscopy_R1_ preprint.pdf
  content_type: application/pdf
  size: 1385605
  md5: ac9f2923c90a66761da07369750e3e37

## Thumbnail

fileset_id: be65dbc9-f11b-46f7-9805-12bb6b215a61
filename: Resolution Improvement of DPC Microscopy_R1_ preprint.pdf